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METHOD FOR DETERMINATION OF RESTRICTION EFFICIENCY OF ENDONUCLEASES MEDIATING DOUBLE-STRAND BREAKS IN DNA TARGET SEQUENCES
METHOD FOR DETERMINATION OF RESTRICTION EFFICIENCY OF ENDONUCLEASES MEDIATING DOUBLE-STRAND BREAKS IN DNA TARGET SEQUENCES
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机译:在DNA靶序列中介导双链断裂的内切核酸酶限制效率的测定方法
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摘要
The invention describes a new method involving bioluminescence resonance energy transfer ratio for determination of restriction efficiency of endonucleases or endonuclease systems comprising a guide-RNA that are used for induction of double-strand breaks at a DNA target sequence of interest for genome editing approaches. The method is suitable for high throughput analyses of potential DNA target sites in combination with different endonuclease-guide-RNA complexes.
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