A method for biotesting of water quality using an obstructive multi-module water quality biotesting apparatus, said apparatus comprising an obstruction index selection device and a water quality testing device; the obstruction index selection device is used for selecting a qualified or effective stepped obstruction level combination, and according to the behaviors of the biological indicators of passing through each obstruction in the effective obstruction level combination after being injected into water to be tested, the water quality testing device tests the water quality of water to be tested. In the method, on the basis of the difference between the behaviors of biological indicators in clean water and polluted water, the distribution areas of biological indicators are counted by establishing a relationship between the degree of water pollution and the bioindicator distribution area, so as to determine the degree of water pollution. The method can accurately and quickly measure the degree of water pollution, without using techniques such as chemical analysis and physical detection, thereby having the advantages of low cost, quick operation, accurate results, and the like.
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