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IN-LINE PRODUCT CONCENTRATION TO REDUCE VOLUMETRIC LOAD FLOW RATE AND INCREASE PRODUCTIVITY OF A BIND AND ELUTE CHROMATOGRAPHY PURIFICATION
IN-LINE PRODUCT CONCENTRATION TO REDUCE VOLUMETRIC LOAD FLOW RATE AND INCREASE PRODUCTIVITY OF A BIND AND ELUTE CHROMATOGRAPHY PURIFICATION
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机译：在线产品浓度降低体积载荷流速并提高粘合和洗脱色谱纯化的生产率
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摘要
Method and system for purifying a sample comprising a biomolecule of interest and impurities, comprising expressing said biomolecule of interest in a bioreactor to form a product sample comprising said biomolecule of interest and impurities; subjecting said product sample to single pass tangential flow filtration to form a concentrated product sample; and subjecting said concentrated product sample to affinity chromatography to remove impurities from said concentrated product sample.
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