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NON-DIAGNOSTIC METHOD FOR MEASURING BRAF GENE V600E MUTATIONS IN COLORECTAL CANCER PATIENT BY MEANS OF CIRCULATING TUMOR CELLS IN PERIPHERAL BLOOD
NON-DIAGNOSTIC METHOD FOR MEASURING BRAF GENE V600E MUTATIONS IN COLORECTAL CANCER PATIENT BY MEANS OF CIRCULATING TUMOR CELLS IN PERIPHERAL BLOOD
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机译：通过循环肿瘤细胞在外周血中测量结肠直肠癌患者BRAF基因V600E突变的非诊断方法
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摘要
A non-diagnostic method for measuring BRAF gene V600E mutations in a colorectal cancer patient by means of circulating tumor cells in peripheral blood, the method primarily comprising collecting peripheral blood, processing the peripheral blood, filtering and enriching circulating tumor cells, and measuring BRAF gene V600E mutation expression in the circulating tumor cells using immunohistochemistry. In the measurement method provided in the present invention, expression of BRAF gene V600E mutations in a colorectal cancer patient can be measured without the use of puncture biopsy to collect a tissue sample. The method is minimally invasive and allows for real-time measurement, and is able to avoid false positive results caused by edge effects possibly produced during a dyeing process, and features good stability, reduction of cell loss, and improved measurement accuracy.
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