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IMMUNOFLUORESCENCE TEST KIT FOR MEASURING PD-L1 GENE MUTATIONS IN CIRCULATING TUMOR CELLS IN PERIPHERAL BLOOD IN NON-SMALL CELL LUNG CANCER PATIENT, AND METHOD FOR SAME
IMMUNOFLUORESCENCE TEST KIT FOR MEASURING PD-L1 GENE MUTATIONS IN CIRCULATING TUMOR CELLS IN PERIPHERAL BLOOD IN NON-SMALL CELL LUNG CANCER PATIENT, AND METHOD FOR SAME
Provided in the present invention are an immunofluorescence test kit for measuring PD-L1 gene mutations in circulating tumor cells in peripheral blood in a non-small cell lung cancer patient, and a method for same, relating to the technical field of molecular biology. The present test kit comprises 45 mL of a diluent, 1 mL of a decoloring solution, 0.5 mL of a dyeing solution A, 1 mL of a dyeing solution B, 200 μL of methanol, 200 μL of 2% PFA, 100 μL of 10% goat serum, 100 μL of a primary antibody working solution, and 100 μL of a secondary antibody working solution, and a DAPI mounting medium. In the measurement method provided in the present invention, expression of PD-L1 in a late-stage or relapsed non-small cell lung cancer patient can be measured without the use of puncture biopsy to collect a tissue sample. The present technology is minimally invasive, and allows for real-time measurement.
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