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IN VITRO FERTILISATION (IVF) EMBRYO VIABILILITY AND QUALITY ASSAY

机译：体外施肥（IVF）胚胎活性和质量测定

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摘要

The present invention is based on evidence of non-contact transfer of embryonic RNA transcripts to endometrium in an in vitro embryo-maternal cross-talk model. RNA is taken up by the endometrial cells and the expression of endogenous transcripts is altered as a result. The effect can be seen in endometrial cells treated with embryo derived extracellular vesicles (EVs) or conditioned medium derived from IVF embryos. RNA from EVs derived from human IVF embryos and conditioned culture media from the human IVF embryos have the potential to change the level of endometrial transcripts. Interestingly, only good- prognosis viable embryos, i.e., capable of producing pregnancies, induced the observed effect while non- competent, e.g., degenerated, embryos failed to initiate any changes. Hence, the capability of inducing a change in RNA transcripts can be used to determine quality of IVF embryos and in predicting pregnancy outcome.

机译：本发明基于在体外胚胎 - 孕妇串扰模型中对胚胎RNA转录物的非接触转移到子宫内膜的证据。通过子宫内膜细胞占据RNA，结果改变内源转录物的表达。在用胚胎衍生的细胞外囊泡（EVS）或衍生自IVF胚胎的条件培养基处理的子宫内膜细胞中可以看到该效果。来自人IVF胚胎和来自人IVF胚胎的人IVF胚胎和调节培养基的RNA具有可能改变子宫内膜转录物的水平。有趣的是，只有良好预后的可行胚胎，即能够产生妊娠，诱导观察到的效果，同时非竞争力，例如退化，胚胎未能发起任何变化。因此，诱导RNA转录物的变化的能力可用于确定IVF胚胎的质量和预测妊娠结果。

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公开/公告号WO2021052996A3

专利类型
公开/公告日2021-09-02

原文格式PDF
申请/专利权人 UNIVERSITY OF TARTU;
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申请/专利号WO2020EP75832
发明设计人 FAZELI ALIREZA;GODAKUMARA GODAGEDARA KASUN MADHURANGA;SALUMETS ANDRES;DISSANAYAKE DISSANAYAKE RALLAGE KEERTHIE CHULANGA;ES-HAGHI MASOUMEH;TROSIN ALEKSANDER;
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申请日2020-09-16
分类号C12Q1/6883;C12Q1/6888;A01K67;
国家 EP
入库时间 2022-08-24 22:26:20

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