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首页> 外国专利> METHOD FOR CARRYING OUT AN ENZYME-LINKED IMMUNOSORBENT ASSAY FOR THE DETECTION OF ANTIBODIES IN A HUMAN BIOLOGICAL SAMPLE SPECIFIC TO THE SARS-COV2 HUMAN CORONAVIRUS, A TEST SYSTEM

METHOD FOR CARRYING OUT AN ENZYME-LINKED IMMUNOSORBENT ASSAY FOR THE DETECTION OF ANTIBODIES IN A HUMAN BIOLOGICAL SAMPLE SPECIFIC TO THE SARS-COV2 HUMAN CORONAVIRUS, A TEST SYSTEM

机译：用于进行酶联免疫吸附试验的方法，用于检测对SARS-COV2人冠状病毒，测试系统特异的人体生物样品中的抗体

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FIELD: medicine.;SUBSTANCE: inventions group relates to the diagnosis of immunity against COVID-19. Disclosed is a method for conducting an enzyme-linked immunosorbent assay for detecting antibodies in a biological sample specific to the human coronavirus SARS-COV2 using a test system, which provides for a reaction for IgM and IgG antibodies separately in different wells in the presence of a recombinant antigen containing the receptor-binding domain of the Spike protein human coronavirus SARS-COV2 (S-RBD), bound to the natural amino-terminal signal peptide and to the C-terminal hexahistidine tag of the Spike protein, while the antigen preparation is added to each well of the 96-well plate, incubated, the solution with the antigen is removed from the plate, washed with PBS-T cells, added to the wells with PBS-T with 4% BSA and incubated for 90 minutes, washed with PBS-T, applied to each well of the plate with antigen solution with PBS-T with 1% BSA, then 8 mcl of blood serum of the test patient, diluted in FSB-T with 1% BSA, is applied, incubated for 1 hour, the wells are washed 4 times with FSB-T, the conjugate of antibodies and enzyme is added, incubated for 40 minutes, washed with FSB-T 6 times, stained using TMB diluted in DMSO, sodium citrate buffer with the addition of 30% hydrogen peroxide, stop the color reaction by adding 0.25 M sulfuric acid and measure the optical density at a wavelength of 450-620 nm. Also disclosed is a test system for carrying out the specified method.;EFFECT: inventions group is suitable for high-precision determination of IgM and IgG antibodies in a biological sample.;2 cl, 6 tbl
机译：田地：医学。物质：发明组涉及对Covid-19免疫的诊断。公开了一种用于使用测试系统进行用于检测对人冠状病毒SARS-COV2特异性的生物样品中的抗体的酶联免疫吸附测定的方法，该试验系统在存在的情况下提供用于IgM和IgG抗体的IgM和IgG抗体的反应含有穗蛋白人冠状病毒SARS-COV2（S-RBD）的受体结合结构域的重组抗原，与天然氨基末端信号肽结合和穗蛋白的C-末端六三氨基标签，同时抗原制剂加入96孔板的每个孔中，孵育，用抗原的溶液从板中除去，用PBS-T细胞洗涤，加入到具有4％BSA的PBS-T中的孔中并孵育90分钟，用PBS-T洗涤，用PBS-T施加到板的每个孔中，用1％BSA，然后在用1％BSA稀释的试验患者的测试患者的8MCL的血清，孵育1小时，井被洗涤4个时间用FSB-T，加入抗体和酶的缀合物，孵育40分钟，用FSB-T洗涤6次，使用在DMSO中稀释的TMB染色，加入30％过氧化氢，停止颜色通过加入0.25M硫酸并测量450-620nm波长的光密度。还公开了用于执行指定方法的测试系统。;效果：发明组适用于生物样品中IgM和IgG抗体的高精度测定。; 2 Cl，6 Tbl

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