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PCR PRIMER PAIR FOR IDENTIFICATION OF OMMASTREPHES BARTRAMII IN THE NORTH PACIFIC OCEAN BASED ON ENVIRONMENTAL DNA, AND METHOD FOR IDENTIFYING OMMASTREPHES BARTRAMII USING THE SAME
PCR PRIMER PAIR FOR IDENTIFICATION OF OMMASTREPHES BARTRAMII IN THE NORTH PACIFIC OCEAN BASED ON ENVIRONMENTAL DNA, AND METHOD FOR IDENTIFYING OMMASTREPHES BARTRAMII USING THE SAME
A primer pair for identification of Ommastrephes bartramii in the North Pacific Ocean based on environmental DNA, and a method for identifying Ommastrephes bartramii using the same. The primer pair consists of a forward primer OMBA-F: 5′-CGAAGGTTAATCTGTCTCCATCT-3′ (SEQ ID NO: 1), and a reverse primer OMBA-R: 5′-CCCAATTAAAATTTATATACCACCACCT-3′ (SEQ ID NO: 2). A DNA molecular marker locus for identifying Ommastrephes bartramii is located at 16S rRNA of Ommastrephes bartramii. The environmental DNA is amplified and sequenced, and if the obtained sequence is completely identical to the sequence of the DNA molecular marker locus, it is indicated that there is Ommastrephes bartramii in the sampling region.
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机译：基于环境DNA的北太平洋识别OmmastRephes Bartramii的底漆对，以及使用该方法识别OmmastreePher Bartramii的方法。 引物对由正向底漆IMBA-F：5'-cgaaggttaatctgtctcatct-3'（SEQ ID NO：1）组成，以及反向引物IMBA-R：5'-CCCAATTAAAATTTATATACCACCCT-3'（SEQ ID NO：2）。 用于识别OmmastRephes Bartramii的DNA分子标记基因座位于16S RRNA的OMMastRephes Bartramii。 扩增和测序环境DNA，如果获得的序列与DNA分子标记基因座的序列完全相同，则表示采样区域中存在Ombstreshshtramii。
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