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首页> 外国专利> METHOD FOR INDIRECT DETERMINATION OF THE NUMBER OF INFECTIOUS DOSES OF RABIES VIRUS STRAIN PB -97 IN RAW MATERIALS FOR ATTENUATED RABIES VACCINE BY REVERSE TRANSCRIPTION AND POLYMERASE CHAIN REACTION IN REAL TIME

METHOD FOR INDIRECT DETERMINATION OF THE NUMBER OF INFECTIOUS DOSES OF RABIES VIRUS STRAIN PB -97 IN RAW MATERIALS FOR ATTENUATED RABIES VACCINE BY REVERSE TRANSCRIPTION AND POLYMERASE CHAIN REACTION IN REAL TIME

机译：间接测定狂犬病病毒菌株PB -97在原料中的传染性剂量菌株PB -97的数量通过实时逆转录和聚合酶链反应减弱狂犬病疫苗

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FIELD: biotechnology. ;SUBSTANCE: invention relates to the field of biotechnology. The invention is a method for indirectly determining the number of infectious doses of rabies virus strain RV-97 in raw materials for attenuated rabies vaccine by reverse transcription and polymerase chain reaction in real time using original specific oligonucleotides and the developed exponential function of the dependence of the threshold amplification cycle and the number of infectious doses of rabies virus strain RV-97. The number of infectious doses of rabies virus is determined during RT-PCR-RT by the value of the threshold amplification cycle. Based on the established value of the threshold amplification cycle (Ct), the value of the number of infectious doses of rabies virus strain RV-97 (NIDRVS RV-97) is calculated using the developed mathematical model: NIDRVS RV-97 =10^(-0,3012⋅Ct+10,2040) (R2=0.9927, E=99.99%). ;EFFECT: present invention makes it possible to quickly and with a high degree of reliability determine the number of infectious doses of rabies virus strain RV-97 in raw materials for an attenuated rabies vaccine based on PCR-RT, followed by the use of the developed mathematical model. ;7 cl, 2 dwg, 8 tbl, 5 ex
机译：领域：生物技术。物质：发明涉及生物技术领域。本发明是一种用于间接测定Rabies病毒菌株RV-97的狂犬病病毒菌株RV-97的数量通过原始的特异性寡核苷酸实时地通过逆转录和聚合酶链反应以及依赖于依赖的幂态函数阈值扩增循环和传染剂量的狂犬病病毒菌株RV-97的数量。在RT-PCR-RT期间通过阈值扩增循环的值确定感染性剂量病毒的数量。基于阈值扩增循环（CT）的成立值，使用开发的数学模型计算了狂犬病病毒菌株RV-97（NIDRVS RV-97）的传染性剂量的数量：NIDRVS RV-97 = 10 ^ （-0,3012˚CT+ 10,2040）（R2 = 0.9927，E = 99.99％）。 ;效果：本发明使得能够快速和高度可靠性确定基于PCR-RT的衰减狂犬病疫苗的狂犬病病毒菌株RV-97的传染性剂量的数量，然后使用发展数学模型。 ; 7 cl，2 dwg，8 tbl，5 ex

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