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首页> 外国专利> SET OF REAGENTS FOR THE DETECTION OF PATHOGENS OF TICK-BORNE BORRELIOSIS

SET OF REAGENTS FOR THE DETECTION OF PATHOGENS OF TICK-BORNE BORRELIOSIS

机译：用于检测蜱传育障碍病原体的试剂

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FIELD: medical biotechnology.;SUBSTANCE: invention relates to the field of medical biotechnology. Invention is a set of reagents for detecting pathogens of tick-borne borreliosis, containing DNA polymerase, buffer for isothermal reaction, the following external and internal primers for detecting Borrelia burgdorferi s DNA. l.:;F3: 5’-GCTGTTGAGCTCCTTCTTG-3’;;B3: 5’-CACCAGCGTCACTTTCAG-3’;;FIP: 5’-TGCAAATCTATTCTCTGGCGAAGGTTGAGCACCTTCTTGAACA-3’;;BIP: 5’-ACAGCAATCGCTTCATCTTGATTCTCAAGCTTCTTGGACC-3’, - new is that it additionally includes the following loop primers for detecting Borrelia burgdorferi s DNA. l.:;LF: 5'-TGCTCCCACATGAACTCTTAA-3 ';;LB: 5'-AAGGAGCTCAGGCTGCTCAGA-3 ',;primers for detecting Borrelia miyamotoi DNA:;F3: 5’-TTCTAACAAAGGACAATATTCCT-3’;;B3: 5’-TTCATTTAAGGGGAAACGATT-3’;;FIP: 5’-CGTTTTCTCTAGCTCGATTGGGAAACACGACCCAGAAATTGACA-3’;;BIP: 5’-CGATATTACGCCACTGACTTCACATGGTTTTTTGTTTTCAGGATCA-3’;;LF: 5’-TGTGCAACATTTGTGGTTG-3’;;LB: 5’- TCACTAAGTCTCAGTGAAAGA-3’,;DNA polymerase Bst 2.0 or Bsm and the corresponding isothermal Bst 2.0 or Bsm buffer, as well as MgSO4 or MgCl2, respectively, a mixture of dNTP nucleotides, betaine, deionized water, positive and negative controls, as well as fluorescent dyes EVA green or ethidium bromide.;EFFECT: invention makes it possible to increase the rapidity and specificity of the analysis for the detection of tick-borne borreliosis, to increase the reliability of the diagnosis by expanding the list of simultaneously determined infectious agents, as well as the possibility of performing the analysis in the field, incl. on whole ticks.;1 cl, 3 dwg, 3 ex
机译：现场：医学生物技术。物质：发明涉及医学生物技术领域。发明是一种用于检测蜱传育缺血蛋白的病原体的一组试剂，含有DNA聚合酶，缓冲液的等温反应，以下外部和内部引物用于检测Borrelia Burgdorferi S DNA。 l :;f3：5'-gctgttgagctcctttctg-3';; b3：5'-caccagccgtcacttcag-3';; fip：5'-tgcaaatctattctctgcgaaggttgagcaccttcttgaaca-3'; bip：5'-acagcaatcgcttcattttgattcaagcttctgacc-3'， - 新是它还包括以下环形引物，用于检测伯克利亚Burgdorferi的DNA。 L.: ;lf：5'-tgctcccacatgaactttaa-3';;磅：5'-aaggagctcaggctgctcaga-3'，;用于检测Borrelia miyamotoi DNA的引物：; f3：5'-ttctaacaaaggacaatattcct-3';; b3：5' TTCATTTAAGGGGAAACGATT-3 ';; FIP：5'-CGTTTTCTCTAGCTCGATTGGGAAACACGACCCAGAAATTGACA-3' ;; BIP：5'-CGATATTACGCCACTGACTTCACATGGTTTTTTGTTTTCAGGATCA-3 ';; LF：5'-TGTGCAACATTTGTGGTTG-3' ;; LB：5'-TCACTAAGTCTCAGTGAAAGA-3' ,; DNA聚合酶BST 2.0或BSM和相应的等温BST 2.0或BSM缓冲液，以及MgSO 4或MgCl 2，分别是DNTP核苷酸，甜菜碱，去离子水，阳性和阴性对照的混合物，以及荧光染料EVA绿色或乙锭溴化物。;效果：发明使得可以通过扩大同时确定的传染性药物的列表以及可能性来增加蜱传育的检测的分析的快速和特异性来增加诊断的可靠性，以及在现场进行分析，包括整个蜱虫。; 1 cl，3 dwg，3前

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