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首页> 外文会议>12th Symposium on Bioluminescence and Chemiluminescence, Apr 5-9, 2002, Robinson College, University of Cambridge, UK >AN ENHANCED CHEMILUMINESCENT IMMUNOASSAY TO DETECT INSULIN
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AN ENHANCED CHEMILUMINESCENT IMMUNOASSAY TO DETECT INSULIN

机译：增强型化学发光免疫分析法检测胰岛素

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摘要

In recent years, although many chemiluminescent clinical immunoassay kits have been widely applied for detection of hormones, the kit for insulin is still not included in most commercial product lists. But it is very important to survey the function of B cells in pancreatic islets in order to diagnose insulinoma and hyperinsulinar obesity, and especially to distinguish the different types of diabetes, which are very common diseases. One of the reasons for this lack is the difficulty in obtaining and selecting suitable pairs of antibodies because insulin itself is the product of hydrolysis of proinsulin and it is difficult to find an anti-insulin antibody that does not have cross-reaction to proinsulin. The insulin molecule is very small, and it is difficult to find two antibodies without binding inhibition for each other, while the competitive assay with single antibody is not sufficiently sensitive for clinical applications. Another reason is the instability of insulin and its short lifetime in sera, and this means it is very difficult to keep the calibrators and samples stable. With a carefully selected pair of antibodies, we have now established an enhanced chemiluminescent immunoassay to detect insulin in human sera.
机译：近年来，尽管许多化学发光临床免疫分析试剂盒已广泛应用于激素检测，但用于胰岛素的试剂盒仍未包括在大多数商业产品清单中。但是，调查胰岛B细胞的功能对于诊断胰岛素瘤和高胰岛素肥胖症，尤其是区分非常常见的不同类型的糖尿病非常重要。这种缺乏的原因之一是难以获得和选择合适的抗体对，因为胰岛素本身是胰岛素原水解的产物，并且很难找到与胰岛素原没有交叉反应的抗胰岛素抗体。胰岛素分子非常小，很难找到没有彼此抑制结合的两种抗体，而具有单种抗体的竞争性检测对于临床应用而言不够灵敏。另一个原因是胰岛素的不稳定性及其在血清中的寿命短，这意味着很难保持校准物和样品的稳定性。通过精心挑选的抗体对，我们现在已经建立了一种增强的化学发光免疫测定法，可以检测人血清中的胰岛素。

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