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首页> 外文会议>12th Symposium on Bioluminescence and Chemiluminescence, Apr 5-9, 2002, Robinson College, University of Cambridge, UK >EVALUATION OF REACTIVE OXYGEN SPECIES PRODUCTION IN KUPFFER CELLS BY CHEMILUMINESCENCE
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EVALUATION OF REACTIVE OXYGEN SPECIES PRODUCTION IN KUPFFER CELLS BY CHEMILUMINESCENCE

机译：化学发光法评估库普弗细胞中活性氧的产生

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摘要

Luminol and lucigenin amplified chemiluminescence is widely used for the sensitive detection of reactive oxygen species (ROS) production in cells such as polymorphonuclear leukocytes and macrophages. However, a few work in this field has been done on Kupffer cells (KC), the resident macrophages of the liver that appear to play an important role in host defence due to their ability to phagocyte bacteria and to detoxify endotoxins. Considerable evidence has shown that ROS production in liver during septic states can contribute to the deterioration of hepatic circulation and that ROS are important mediators in liver injury. Therefore, the study of ROS production by Kupffer cells during infectious states is important to elucidate the mechanisms of the onset of liver damage. At present, there are not studies in the literature about the ability of the spirochete to induce the production of ROS by Kupffer cells. Therefore, in this work we used luminol amplified chemiluminescence to study ROS production by isolated rat Kupffer cells in the presence of three spirochetes: Borrelia garinii (the aethiological agent of Lyme disease), Leptospira interrogans (the causative agent of Leptospirosis), and Treponema pallidum (the cause of Syphilis).
机译：鲁米诺和光泽精放大的化学发光方法广泛用于灵敏检测细胞（如多形核白细胞和巨噬细胞）中的活性氧（ROS）产生。但是，该领域的一些工作已在库普弗细胞（KC）上完成，库普弗细胞是肝脏的常驻巨噬细胞，由于其具有吞噬细胞细菌和解毒内毒素的能力，因此在宿主防御中起着重要作用。大量证据表明，败血症状态下肝脏中ROS的产生可导致肝循环的恶化，并且ROS是肝损伤的重要介质。因此，研究Kupffer细胞在感染状态下产生ROS的方法对于阐明肝损害发作的机制非常重要。目前，在文献中还没有关于螺旋体诱导库普弗细胞产生ROS的能力的研究。因此，在这项工作中，我们使用了鲁米诺扩增的化学发光来研究分离的大鼠Kupffer细胞在以下三种螺旋体的存在下产生的ROS：螺旋体疏螺旋体（莱姆病的病原体），问号钩端螺旋体（钩端螺旋体病的病原体）和苍白螺旋体（梅毒的病因）。

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