CATALYTIC PROPERTIES AND BIOLUMINESCENCE SPECTRA OF RECOMBINANT FIREFLY LUCIFERASE LUCIOLA MINGRELICA WITH POINT MUTATIONS OUT OF THE ENZYME ACTIVE SITE

机译：酶活性部位外点突变的重组萤光酶LUCIOLA MINGRELICA的催化性能和生物发光光谱

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The His433Tyr mutation in Luciola cruciata and Hotaria parvula firefly luciferases resulted in bioluminescence color changes from 570 to 610 nm. It means that the highly conservative His433 residue located out of the luciferase active site plays a very important role in enzyme function. The goal of this work was to construct mutants His433Asn and His433Ser for Luciola mingrelica firefly luciferase, which has high homology with luciferases indicated above. We studied catalytic properties of the enzyme mutant forms and their bioluminescence spectra. Analysis of the data obtained permitted us to elucidate the mechanism of the influence of the His433 residue on the luciferase active site.

机译：Luciola cruciata和Hotaria parvula萤火虫荧光素酶中的His433Tyr突变导致生物发光颜色从570 nm变为610 nm。这意味着位于荧光素酶活性位点之外的高度保守的His433残基在酶功能中起着非常重要的作用。这项工作的目的是构建与上述萤光素酶具有高度同源性的Luciola mingrelica萤火虫萤光素酶的突变体His433Asn和His433Ser。我们研究了酶突变体形式的催化特性及其生物发光光谱。对获得的数据的分析使我们能够阐明His433残基对荧光素酶活性位点的影响机理。

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《12th Symposium on Bioluminescence and Chemiluminescence, Apr 5-9, 2002, Robinson College, University of Cambridge, UK》|2002年|p.45-48|共4页
会议地点 Cambridge(GB)
作者
LG MALOSHENOK; NN UGAROVA;
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作者单位

Dept of Chemistry, Lomonosov Moscow State University, Moscow 119899, Russia;

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原文格式 PDF
正文语种 eng
中图分类生物物理学;
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专利

1. Catalytic properties and bioluminescence spectra of firefly Luciola mingrelica wiht point mutations exterior to the active site [J] . L.G.Maloshenok, I.V.Uporov, N.N.Ugarova Moscow University Chemistry Bulletin . 2002,第6期

机译：萤火虫Luciola mingrelica具有活性位点外部突变的催化特性和生物发光光谱
2. Random Mutagenesis of Luciola mingrelica Firefly Luciferase. Mutant Enzymes with Bioluminescence Spectra Showing Low pH Sensitivity [J] . M. I. Koksharov, N. N. Ugarova Biochemistry . 2008,第8期

机译：Luciola mingrelica萤火虫荧光素酶的随机诱变。具有生物发光光谱的突变酶显示出低pH敏感性
3. Active site mutants of Escherichia coli dethiobiotin synthetase: effects of mutations on enzyme catalytic and structural properties. [J] . Yang G, Sandalova T, Lohman K, Biochemistry . 1997,第16期

机译：大肠杆菌脱硫生物素合成酶的活性位点突变体：突变对酶催化和结构性质的影响。
4. CATALYTIC PROPERTIES AND BIOLUMINESCENCE SPECTRA OF RECOMBINANT FIREFLY LUCIFERASE LUCIOLA MINGRELICA WITH POINT MUTATIONS OUT OF THE ENZYME ACTIVE SITE [C] . LG MALOSHENOK, NN UGAROVA Symposium on bioluminescence and chemiluminescence . 2002

机译：重组萤火虫荧光素酶Luciola mingrelica的催化性质和生物发光光谱用点突变从酶活性位点出来
5. The active-site cysteines of firefly luciferase: Chemical modification and mutagenesis studies. [D] . Vellom, Daniel Charles. 1990

机译：萤火虫荧光素酶的活性位点半胱氨酸：化学修饰和诱变研究。
6. An enhanced chimeric firefly luciferase-inspired enzyme for ATP detection and bioluminescence reporter and imaging applications [O] . Bruce R. Branchini, Tara L. Southworth, Danielle M. Fontaine, -1

机译：一种增强的嵌合萤火虫荧光素酶启发性酶用于ATP检测和生物发光报告基因及成像应用
7. Glu311 and Arg337 Stabilize a Closed Active-site Conformation and Provide a Critical Catalytic Base and Countercation for Green Bioluminescence in Beetle Luciferases [O] . -1

机译：Glu311和Arg337稳定封闭的有效部位构象，并提供甲虫荧光素酶在甲虫荧光素中的绿色生物发光的关键催化基础和反应
8. Sensitive Dual Color in vivo Bioluminescence Imaging Using a New Red Codon Optimized Firefly Luciferase and a Green Click Beetle Luciferase [R] . Mezzanotte, L., Que, I., Kaijzel, E., 2011

机译：敏感的双色体内生物发光成像使用新的红色密码子优化的萤火虫荧光素酶和绿色点击甲虫萤光素酶

CATALYTIC PROPERTIES AND BIOLUMINESCENCE SPECTRA OF RECOMBINANT FIREFLY LUCIFERASE LUCIOLA MINGRELICA WITH POINT MUTATIONS OUT OF THE ENZYME ACTIVE SITE

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