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首页> 外文会议>12th Symposium on Bioluminescence and Chemiluminescence, Apr 5-9, 2002, Robinson College, University of Cambridge, UK >A NOVEL BIOLUMINESCENT CYCLING ASSAY FOR ATP AND AMP USING PYRUVATE ORTHOPHOSPHATE DIKINASE
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A NOVEL BIOLUMINESCENT CYCLING ASSAY FOR ATP AND AMP USING PYRUVATE ORTHOPHOSPHATE DIKINASE

机译：丙酮酸邻苯二甲酸二激酶对ATP和AMP的新型生物发光循环分析

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摘要

In any food-processing environment, food residues can encourage and facilitate the survival and growth of microorganism by providing a nutritious medium for growth and protection from disinfectants. In order to prevent contamination of the product, it is important to keep food contact surfaces clean. Several hygiene monitoring systems for determining the cleanliness of surfaces were developed. Above all, the adenosine triphosphate (ATP) method is recommended due to ATP being widely found in food residues and microorganisms. However, in some food residues the ATP method shows insufficient sensitivity, especially processed foods. In order to improve the sensitivity, we have developed a novel bioluminescent enzymatic cycling system using pyruvate orthophosphate dikinase (PPDK) (ATP-PPDK method) (Fig. 1). PPDK catalyses the formation of ATP from AMP, pyrophosphate (PPi), and phosphoenolpyruvate (PEP). This system enables the detection of the combined total ATP and AMP with a high degree of consistency and higher bioluminescence when compared to existing ATP methods. We have applied this system to hygiene monitoring and have developed a novel hygiene monitoring device "LuciPac W" and photodiode luminometer "Lumitester PD-10".
机译：在任何食品加工环境中，食物残渣都可以通过提供营养培养基来生长和保护免受消毒剂的刺激，从而促进和促进微生物的存活和生长。为了防止产品污染，保持食物接触表面清洁很重要。开发了几种用于确定表面清洁度的卫生监测系统。最重要的是，由于在食物残渣和微生物中广泛发现ATP，因此推荐使用三磷酸腺苷（ATP）方法。但是，在某些食品残留物中，ATP方法显示的灵敏度不足，尤其是加工食品。为了提高灵敏度，我们使用丙酮酸正磷酸二激酶（PPDK）（ATP-PPDK方法）开发了一种新型的生物发光酶促循环系统（图1）。 PPDK催化由AMP，焦磷酸盐（PPi）和磷酸烯醇丙酮酸（PEP）形成ATP。与现有的ATP方法相比，该系统能够以高度的一致性和更高的生物发光检测总的ATP和AMP。我们已将该系统应用于卫生监测，并开发了新型卫生监测设备“ LuciPac W”和光电二极管发光计“ Lumitester PD-10”。

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