USE OF BIOLUMINESCENCE FOR THE EVALUATION OF AFFINITY CONSTANTS FOR BACTERIAL CELL-ANTIBODY INTERACTIONS

机译：利用生物发光法评估细菌细胞-抗体相互作用的亲和力常数

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The bioluminescent affinity test presented here can be completed within 1 to 1 1/2 h and gives an estimate of the functional affinity of antibodies against live bacterial cells such as Salmonella enteritidis. The use of bacteria with bioluminescent and possibly fluorescent phenotypes to determine the affinity constants of antibodies, is a rapid and easy approach for selecting high affinity and specific antibodies for use in detection assays.

机译：此处介绍的生物发光亲和力测试可以在1至1 1/2个小时内完成，并可以估算出抗体对活细菌细胞（如肠炎沙门氏菌）的功能亲和力。使用具有生物发光和可能的荧光表型的细菌来确定抗体的亲和常数，是一种快速简便的方法，用于选择高亲和力和特异性抗体以用于检测分析。

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来源
《12th Symposium on Bioluminescence and Chemiluminescence, Apr 5-9, 2002, Robinson College, University of Cambridge, UK》|2002年|p.435-438|共4页
会议地点 Cambridge(GB)
作者
D YOUNG; MW GRIFFITHS; L BROVKO;
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作者单位

Dept of Food Science, University of Guelph, Guelph ON N1G 2W1, Canada;

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原文格式 PDF
正文语种 eng
中图分类生物物理学;
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1. Measurement of the dissociation-equilibrium constants for low affinity antibiotic binding interaction with bacterial ribosomes by the T_2 (CPMG) and line-broadening methods [J] . L. Verdier, J. Gharbi-Benarous, G. Bertho, Journal de Chimie Physique et de Physico-Chimie Biologique . 1999,第9a10期

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2. Determination of Rate Constants and Equilibrium Constants for Solution-Phase Drug-Protein Interactions by Ultrafast Affinity Extraction [J] . Xiwei Zheng, Zhao Li, Maria I. Podariu, Analytical chemistry . 2014,第13期

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3. Evaluation of Taylor dispersion injections: Determining kinetic/affinity interaction constants and diffusion coefficients in label-free biosensing [J] . Quinn J.G. Analytical Biochemistry: An International Journal of Analytical and Preparative Methods . 2012,第2期

机译：泰勒分散体注射的评估：确定无标记生物传感中的动力学/亲和力相互作用常数和扩散系数
4. USE OF BIOLUMINESCENCE FOR THE EVALUATION OF AFFINITY CONSTANTS FOR BACTERIAL CELL-ANTIBODY INTERACTIONS [C] . D YOUNG, MW GRIFFITHS, L BROVKO Symposium on bioluminescence and chemiluminescence . 2002

机译：使用生物发光进行细菌细胞 - 抗体相互作用的亲和常数评价
5. Generation of fluorescent strains to evaluate probiotic and pathogenic bacterial interactions in larvae of the eastern oyster, Crassostrea virginica. [D] . Oates, Lauren M. 2013

机译：荧光菌株的产生，以评估东部牡蛎Crassostrea virginica幼虫中益生菌和致病细菌的相互作用。
6. Evaluation of equilibrium constants for the interaction of lactate dehydrogenase isoenzymes with reduced nicotinamide-adenine dinucleotide by affinity chromatography. [O] . R I Brinkworth, C J Masters, D J Winzor 1975

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7. Determination of Rate Constants and Equilibrium Constants for Solution-Phase Drug–Protein Interactions by Ultrafast Affinity Extraction [O] . Xiwei Zheng, Zhao Li, Maria I. Podariu, 2014

机译：超快亲和提取测定溶液相药 - 蛋白质相互作用的速率常数和平衡常数

USE OF BIOLUMINESCENCE FOR THE EVALUATION OF AFFINITY CONSTANTS FOR BACTERIAL CELL-ANTIBODY INTERACTIONS

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