Abstract: Biomedical optics involves with individual index of refraction of biological tissue. A new method to measure refractive index and the accuracy analysis as well is presented. The characteristic includes the incident angle is changed with parallel movement of incident beam and the refractive index of sample is determined linearly. A narrow laser beam and quarter-cylindrical lens in contact with a sample are applied in the apparatus. The device can provide the index for various tissue samples with no complex mathematical analysis and the calibration of device is simple. A number of indices for human tissue have been determined at 632.8 nm.!6
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