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首页> 外文会议>Nano Science and Technology Institute(NSTI) Nanotechnology Conference and Trade Show(NSTI Nanotech 2006) vol.2 >Integrated Microfluidic Device with an Electroplated Palladium Decoupler for Electrochemical Detection of the 8-hydroxy-deoxyguanosine (8-OH-dG) DNA Adduct
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Integrated Microfluidic Device with an Electroplated Palladium Decoupler for Electrochemical Detection of the 8-hydroxy-deoxyguanosine (8-OH-dG) DNA Adduct

机译：具有电镀钯解耦器的集成微流控装置，用于电化学检测8-羟基-脱氧鸟苷（8-OH-dG）DNA加合物

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摘要

8-hydroxy-deoxyguanosine DNA adduct (8-OH-dG) is one of the most frequently used biomarkers reporting on the oxidative stress that leads to DNA damage. More sensitive and reliable microfluidic devices are needed for the detection of these biomarkers of interest. We have developed a capillary electrophoresis based microfluidic device with an electroplated palladium decoupler that provides significantly improved detection limit, separation efficiency, and resolving power. The PDMS/glass hybrid device has fully integrated gold microelectrodes covered in situ with palladium nanoparticles using an electroplating technique. The quality of the electroplated palladium particles were evaluated electrochemically and via SEM imaging. The performance of the device was tested and evaluated with different buffer systems, pH's and electric field strengths. The results showed that this device has significantly improved resolving power, even at separation electric field strengths as high as 600 V/cm. The detection limit for the 8-OH-dG adduct is about 20 attomoles; the concentration limit is on the order of 100 nM (S/R =3). A linear response is reported for both 8-OH-dG and dG in the range from 100 nM to 150 μM (～100 pA/μM) with separation efficiencies of ～120,000 - 170,000 plates/m.
机译：8-羟基-脱氧鸟苷DNA加合物（8-OH-dG）是最常用的生物标志物之一，报告了导致DNA损伤的氧化应激。需要更灵敏和可靠的微流控设备来检测这些感兴趣的生物标记。我们已经开发了一种基于毛细管电泳的微流控设备，该设备具有电镀钯解耦器，可显着提高检测限，分离效率和分离能力。 PDMS /玻璃混合设备具有完全集成的金微电极，该微电极使用电镀技术原位覆盖有钯纳米颗粒。电化学评估电镀钯颗粒的质量，并通过SEM成像。使用不同的缓冲液系统，pH值和电场强度对设备的性能进行了测试和评估。结果表明，即使在分离电场强度高达600 V / cm的情况下，该设备也具有显着提高的分辨能力。 8-OH-dG加合物的检出限为约20个小原子。浓度极限约为100 nM（S / R = 3）。据报道，8-OH-dG和dG的线性响应范围为100 nM至150μM（〜100 pA /μM），分离效率为〜120,000-170,000板/ m。

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