• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>2010 International Conference on Bioinformatics and Biomedical Technology (ICBBT 2010) >Comparison of Tn1546 element of vancomycin resistant Staphylococcus aureus isolated from burned patients in Sulaimani hospital
【24h】

Comparison of Tn1546 element of vancomycin resistant Staphylococcus aureus isolated from burned patients in Sulaimani hospital

机译：苏莱曼尼医院烧伤患者分离出的耐万古霉素金黄色葡萄球菌Tn1546元件的比较

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

little is known about vancomycin-resistant Staphylococci in Iraq. Staphylococcus aureus, a major cause of potentially life-threatening infections acquired in health care settings and in the community, has developed resistance to most classes of antimicrobial agents soon after their introduction into clinical use. In many health care institutions around the world, glycopeptides such as vancomycin provide effective therapy against most strains of multidrug-resistant S. aureus, including MRSA. In 2008, the first nine clinical isolates of vancomycin-resistant Staphylococcus aureus (VRSA) containing vanA gene with variable vancomycin MICs were recovered in Sulaimani Emergency hospital for war victims/Sulaimani/ Kurdistan region of Iraq. With PCR and DNA sequence analysis, the Tn1546 elements, a mobile genetic element that encodes vancomycin resistance proteins in enterococci and staphylococci, amplified and the sequences of which was compared to the prototype Tn1546 element from enterococci and also to the previously sequenced Tn1546 of VRSA recorded in gene bank/ NCBI, the Sulaimani VRSA Tn1546 element was different by distinct modifications in nucleotide sequences and showed partial homology with an enterococcal transposone Tn1546-like element. These differences in the Tn1546 elements explain various expression level of VanA gene which indicated by vancomycin MICs. This is believed to be the first Iraqi molecular study on S. aureus isolate that has been shown to be vancomycin-resistant, due to a Tn1546 transposone analogue..
机译：对于伊拉克耐万古霉素的葡萄球菌知之甚少。金黄色葡萄球菌是在医疗机构和社区中引起潜在威胁生命的感染的主要原因，在将其引入临床后不久就对大多数种类的抗菌剂产生了耐药性。在世界各地的许多医疗机构中，诸如万古霉素之类的糖肽均可对大多数具有多重耐药性的金黄色葡萄球菌菌株（包括MRSA）提供有效的疗法。 2008年，在伊拉克苏拉曼尼急诊医院/苏拉曼尼/库尔德斯坦地区的苏拉马尼急诊医院，回收了前9株含有vanA基因且具有可变万古霉素MIC的耐万古霉素金黄色葡萄球菌（VRSA）。通过PCR和DNA序列分析，扩增了Tn1546元件（一种编码肠球菌和葡萄球菌中万古霉素抗性蛋白的移动遗传元件），并将其序列与来自肠球菌的原型Tn1546元件进行了比较，还与先前记录的VRSA的Tn1546进行了比较在基因库/ NCBI中，Sulaimani VRSA Tn1546元素因核苷酸序列的不同修饰而有所不同，并显示出与肠球菌转座子Tn1546-样元素的部分同源性。 Tn1546元素的这些差异解释了万古霉素MICs指示的VanA基因的各种表达水平。据信，这是伊拉克对金黄色葡萄球菌分离物的第一个分子研究，由于Tn1546转座子类似物，已被证明对万古霉素具有抗性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号