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A segmentation method for microscope images of BY-2 tobacco cells in suspension cultures

机译:悬浮培养中BY-2烟草细胞显微图像的分割方法

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This paper describes a segmentation method of BY-2 tobacco cells proceeded from suspension cultures. The method separates foreground objects by the detection of their boundaries and then eliminates cell clumps and artefacts based on their different inside brightness and skeleton structures. Single cell objects are restored by the thickening of binary cell residues remaining after morphological operation of cleaning the holes under the cell chain masks. Experimental results of the segmentation for a series of BY-2 images as well as cell enumeration and segmentation errors are presented.
机译:本文介绍了从悬浮培养中分离出BY-2烟草细胞的方法。该方法通过检测前景物体的边界来分离前景物体,然后基于它们不同的内部亮度和骨骼结构消除细胞团块和伪像。在清洁细胞链掩膜下的孔的形态学操作后,残留的二元细胞残留物变稠,可以恢复单个细胞对象。提出了一系列BY-2图像分割的实验结果,以及细胞枚举和分割误差。

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