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首页> 外文会议>International Conference on Computer Science and Information Processing;CSIP 2012 >CDNA, genomic sequence cloning and sequence analysis of ribosomal protein L14 gene(rpL14) from Ailuropoda melanoleuca
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CDNA, genomic sequence cloning and sequence analysis of ribosomal protein L14 gene(rpL14) from Ailuropoda melanoleuca

机译：黑线虫的核糖体蛋白L14基因（rpL14）的CDNA，基因组序列克隆和序列分析

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摘要

To explore the structure characteristic of rpL14 gene of A. melanoleuca and the differences among other species reported, we have designed primers based on the known information of rpL14 gene. Then, the cDNA and the genomic sequence of rpL14 gene were cloned successfully from A. melanoleuca by RT-PCR and PCR strategy respectively, and followed by sequencing and analyzing. The result indicated that the length of cDNA fragment cloned from A. melanoleuca is 700 bp in size, containing an open reading frame of 636bp. Predicted protein was composed of 211 amino acids with an estimated molecular weight of 23.06 kD and PI of 11.70. The length of the genomic sequence is 3292 bp, which was found to have six exons and five introns. Alignment analysis indicates that rpL14 gene and deduced amino acid sequence are highly similarity with reported species, including Homo sapiens, Bos taurus, Rattus norvegicus, Mus musculus and Sus scrofa. The homologies for nucleotide sequences of A. melanoleuca rpL14 with this five species are 89.51%, 89.77%, 85.43%, 85.17% and 89.25%, while the homologies for amino acid sequences are 79.07%, 90.19 %, 86.45%, 84.79% and 94.37%. Topology predicted shows that six different patterns were found in the RPL14 protein of A. melanoleuca. The rpL14 gene of the A. melanoleuca was studied in this paper, which provided scientific information to enrich the rpL14 gene database.
机译：为了探究黑毛拟南芥rpL14基因的结构特征和报道的其他物种之间的差异，我们根据rpL14基因的已知信息设计了引物。然后，分别通过RT-PCR和PCR方法成功地从黑麦草中克隆了rpL14基因的cDNA和基因组序列，并进行了测序和分析。结果表明，从黑毛油曲霉克隆的cDNA片段的长度为700bp，包含一个636bp的开放阅读框。预测的蛋白质由211个氨基酸组成，估计的分子量为23.06 kD，PI为11.70。基因组序列的长度为3292 bp，发现有六个外显子和五个内含子。比对分析表明，rpL14基因和推导的氨基酸序列与报道的物种高度相似，包括智人，金牛座，褐家鼠，小家鼠和田鼠。与这五个物种的黑色链球菌rpL14的核苷酸序列同源性为89.51％，89.77％，85.43％，85.17％和89.25％，而氨基酸序列的同源性为79.07％，90.19％，86.45％，84.79％和94.37％。预测的拓扑表明，在黑麦草的RPL14蛋白中发现了六种不同的模式。本文研究了黑皮草的rpL14基因，为丰富rpL14基因数据库提供了科学信息。

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