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首页> 外文会议>2014 40th Annual Northeast Bioengineering Conference >Variations in moisture retention of contact-printed soil bacteria surface colonies using confocal microscopy
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Variations in moisture retention of contact-printed soil bacteria surface colonies using confocal microscopy

机译：共聚焦显微镜观察接触印刷的土壤细菌表面菌落的保水率变化

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The retention of soil moisture enables vegetation to remain viable between rainfall or irrigation events. Microbial production of extracellular polymeric substances (EPS) can influence soil moisture in two ways: first, by retaining moisture directly in the carbohydrate-rich hydrogel material itself; and second, by promoting an aggregated structure of soil grains. In whole soils, it can be difficult to decouple these two effects. The objective of this study was to measure the direct moisture-retaining properties of extracellular polymeric substances produced by a common soil isolate. Here, water retention in biofilm-like colonies of GFP-expressing Sinorhiobium meliloti were measured under controlled relative humidity as a volume change with time using confocal microscopy. S. meliloti strains with muccoid (EPS+) and non-muccoid (EPS-) traits were compared. Methods to collect and contact-print biofilm-like colonies on a glass substrate and to quantify volume changes with time were developed. The results show EPS+ biofilms are more resistant to drying than are EPS-biofilms. Under identical atmospheric conditions, similarly-sized EPS-biofilms decreased in volume to 17% of their original size, while EPS+ biofilms retained 50% of their original volume. This work may enable development of environmental biotechnologies to improve moisture retention and enhance agricultural productivity.
机译：保持土壤水分可使植被在降雨或灌溉事件之间保持活力。微生物产生的细胞外聚合物质（EPS）可以通过两种方式影响土壤湿度：第一，通过将水分直接保留在富含碳水化合物的水凝胶材料本身中来解决；第二，通过促进土壤颗粒的聚集结构。在整个土壤中，可能很难将这两种效应分离。这项研究的目的是测量常见土壤分离物产生的细胞外聚合物的直接保水性能。在这里，使用共聚焦显微镜在受控的相对湿度下，随着时间的变化，测量了表达GFP的Sinorhiobium meliloti的生物膜样菌落中的保水量。比较了具有粘液样（EPS +）和非粘液样（EPS-）特性的苜蓿链球菌菌株。开发了在玻璃基板上收集和接触印刷生物膜样菌落并量化随时间变化的方法。结果表明，EPS +生物膜比EPS生物膜更耐干燥。在相同的大气条件下，尺寸相似的EPS生物膜的体积减小至其原始大小的17％，而EPS +生物膜保留其原始体积的50％。这项工作可以促进环境生物技术的发展，以改善水分保持能力并提高农业生产率。

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