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Extraction and analysis of neuron firing signals from deep cortical video microscopy

机译:深层皮质视频显微镜对神经元放电信号的提取与分析

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We introduce a method for extracting and analyzing neuronal activity time signals from video of the cortex of a live animal. The signals correspond to the firing activity of individual cortical neurons. Activity signals are based on the changing fluorescence of calcium indicators in the cells over time. We propose a cell segmentation method that relies on a user-specified center point, from which the signal extraction method proceeds. A stabilization approach is used to reduce tissue motion in the video. The extracted signal is then processed to flatten the baseline and detect action potentials. We show results from applying the method to a cortical video of a live mouse.
机译:我们介绍了一种从活动物的皮质视频中提取和分析神经元活动时间信号的方法。该信号对应于单个皮质神经元的激发活性。活性信号基于细胞中钙指示剂随时间变化的荧光。我们提出了一种基于用户指定中心点的细胞分割方法,信号提取方法将从该中心点继续进行。稳定方法用于减少视频中的组织运动。然后对提取的信号进行处理以使基线变平并检测动作电位。我们展示了将该方法应用于活体小鼠皮层视频的结果。

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