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Extraction and analysis of neuron firing signals from deep cortical video microscopy

机译:深皮瓣视频显微镜神经元烧制信号的提取与分析

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We introduce a method for extracting and analyzing neuronal activity time signals from video of the cortex of a live animal. The signals correspond to the firing activity of individual cortical neurons. Activity signals are based on the changing fluorescence of calcium indicators in the cells over time. We propose a cell segmentation method that relies on a user-specified center point, from which the signal extraction method proceeds. A stabilization approach is used to reduce tissue motion in the video. The extracted signal is then processed to flatten the baseline and detect action potentials. We show results from applying the method to a cortical video of a live mouse.
机译:我们介绍一种用于从活动物的皮质视频中提取和分析神经元活动时间信号的方法。信号对应于单个皮质神经元的烧制活性。活性信号基于细胞中钙指示剂的荧光随时间的变化。我们提出了一种依赖于用户指定的中心点的小区分割方法,信号提取方法继续。稳定方法用于减少视频中的组织运动。然后将提取的信号加工以平整基线并检测动作电位。我们展示了将方法应用于实时鼠标的皮质视频。

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