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首页> 外文会议>2014 International Conference on Science Engineering and Management Research >Clinical validation of vaccines against hemolytic uremic syndrome
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Clinical validation of vaccines against hemolytic uremic syndrome

机译：溶血性尿毒症综合征疫苗的临床验证

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To compare the efficacy of a whole-cell vaccine and a toxoid vaccine prepared for Escherichia coli O157:H7 strain using rats as animal models. Methods: Isolation and identification of study strain from samples such as ground beef, chicken intestine, raw milk and pasteurized milk was followed by confirmation of five selected virulence genes by conventional PCR amplification. Whole cell vaccine and a toxoid vaccine, prepared as protein produced out of stx1 gene variant from an isolate strain of study were subjected to its efficacy test using rat models after obtaining proper ethical clearance. After challenging with ATCC and isolates, screening performed by histology in liver and kidney on test rats as well as on control rats. Results: Normal histology was seen in liver and kidney specimens of vaccinated and challenged rat groups as well as control un-inoculated unvaccinated rat groups with very few exceptional lesions. However, severe toxic evidences were observed in rat groups which were unvaccinated and challenged with pathogenic strains. Conclusions: Vaccine is believed to have lost its pathogenicity to a greater extent as validated by animal studies. When comparison was made between whole cell vaccine and toxoid, whole cell vaccine had greater extent of efficiency than toxoid.
机译：为了比较以大鼠为动物模型为大肠杆菌O157：H7菌株制备的全细胞疫苗和类毒素疫苗的功效。方法：从碎牛肉，鸡肠，生奶和巴氏杀菌奶等样品中分离和鉴定研究菌株，然后通过常规PCR扩增确认五个选定的毒力基因。全细胞疫苗和类毒素疫苗，是从分离的研究菌株的stx1基因变异体生产的蛋白质中制备的，在获得适当的伦理审查后，使用大鼠模型对其功效进行了测试。用ATCC和分离物攻击后，通过肝组织和肾脏组织学方法对试验大鼠和对照大鼠进行筛选。结果：在接种过疫苗的和攻击过的大鼠组的肝脏和肾脏标本中，以及未感染且未接种过空白对照组的对照组中，肝脏和肾脏的标本均正常。但是，在未接种疫苗并受到致病株攻击的大鼠组中，观察到了严重的毒性证据。结论：据动物研究证实，疫苗被认为在更大程度上丧失了其致病性。当对全细胞疫苗和类毒素进行比较时，全细胞疫苗比类毒素具有更高的效率。

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