• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>2014 International Symposium on Optomechatronic Technologies >Rapid Multiplexed Imaging of Cell-Surface Cancer Biomarkers in Fresh Tissues with Targeted SERS Nanoparticles
【24h】

Rapid Multiplexed Imaging of Cell-Surface Cancer Biomarkers in Fresh Tissues with Targeted SERS Nanoparticles

机译：靶向SERS纳米颗粒的新鲜组织中细胞表面癌生物标记物的快速多路复用成像

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Our lab is developing miniature Raman imaging systems and topical-staining protocols to rapidly image cell-surface biomarkers in fresh tissues. In particular, this work employs targeted surface-enhanced Raman scattering (SERS) nanoparticles (NPs) to enable the sensitive and multiplexed detection of a large number of cell-surface biomarkers of cancer. The SERS NPs were functionalized with different targeting antibodies, and their biomarker detection capability was investigated via in vitro and ex vivo experiments with cells and tissues. Here, we design SERS NPs to specifically target the cancer biomarker EGFR upon topical application on cells and tissues. In vitro flow cytometry with fluorescent SERS NPs reveals a high ratio of specific versus nonspecific binding for the tumor cell lines A431 (skin cancer), U251 (glioma) and SkBr3 (breast cancer). For tissue imaging, we have developed a fiber-optic-based spectral detection probe, with 785-nm laser illumination, for rapid detection of SERS NPs with sub-millimeter spatial resolution. Based on the spectral detection probe, multiple imaging systems were customized for rapid tissue phenotyping such as a comprehensive rotational scanning endoscope for in vivo imaging of the rat esophagus and a raster-scanning device for intraoperative imaging of breast tissue margins. Ex vivo experiments were performed to develop a strategy for the rapid detection of multiple cell-surface biomarkers following a brief (5-10 min) topical application of SERS NPs on tissues. By developing high-affinity targeted SERS NPs, sensitive spectral-imaging devices, and an optimized topical-delivery protocol, we demonstrate a ratio metric method to rapidly quantify the specific binding of biomarker-targeted NPs on fresh tissues, thereby eliminating the ambiguities that often arise due to nonspecific sources of contrast. These tools will enable multiplexed molecular imaging for the early detection of epithelial cancers, rapid surgical guidance, and moni- oring the molecular response to treatments.
机译：我们的实验室正在开发微型拉曼成像系统和局部染色方案，以对新鲜组织中的细胞表面生物标记物快速成像。特别是，这项工作采用了靶向表面增强拉曼散射（SERS）纳米颗粒（NPs），能够对多种癌症细胞表面生物标记物进行灵敏和多重检测。用不同的靶向抗体对SERS NP进行功能化，并通过细胞和组织的体外和离体实验研究了它们的生物标志物检测能力。在这里，我们设计SERS NPs在细胞和组织上局部应用后，特异性靶向癌症生物标志物EGFR。用荧光SERS NPs进行的体外流式细胞术揭示了肿瘤细胞系A431（皮肤癌），U251（神经胶质瘤）和SkBr3（乳腺癌）的特异性与非特异性结合率很高。对于组织成像，我们开发了一种基于光纤的光谱检测探头，具有785 nm激光照射，可用于以亚毫米空间分辨率快速检测SERS NP。基于光谱检测探针，定制了多种成像系统用于快速组织表型分析，例如用于大鼠食管体内成像的综合旋转扫描内窥镜和用于术中乳腺组织边缘成像的光栅扫描装置。进行离体实验以开发在组织上短暂（5-10分钟）局部应用SERS NP后快速检测多种细胞表面生物标志物的策略。通过开发高亲和力靶向SERS NP，敏感的光谱成像设备和优化的局部给药方案，我们证明了一种比率度量方法可以快速定量以生物标记物为靶标的NP在新鲜组织上的特异性结合，从而消除了经常出现的歧义由于非特定的对比来源而出现。这些工具将使多重分子成像成为可能，以便及早发现上皮癌，迅速进行手术指导并监测对治疗的分子反应。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号