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首页> 外文会议>221st National Meeting of the American Chemical Society Apr 1-5, 2001 San Diego, California >The Role of Enzymes and Mediators in White-Rot Fungal Degradation of Lignocellulose
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The Role of Enzymes and Mediators in White-Rot Fungal Degradation of Lignocellulose

机译：酶和介体在木质纤维素白腐菌降解中的作用

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摘要

White-rot fungi can selectively and efficiently degrade lignin through the action of secreted enzymes. Major lignin-degrading enzymes include lignin peroxidase (LiP), manganese peroxidase (MnP) and laccase. LiP and MnP require hydrogen peroxide (H_2O_2) for their activities, but they are inactivated by a high concentration of H_2O_2. There are various H_2O_2-producing enzymes in white-rot fungi. LiP is able to oxidize both phenolic and non-phenolic lignin substructures, whereas MnP and laccase are only able to degrade phenolic lignin substructures. An unsaturated fatty acid or a thio-containing compound enables MnP to degrade non-phenolic lignin substructures. Several in vitro studies reveal that some enzymes can synergistically degrade lignin. As lignin-degrading enzymes are too bulky to penetrate plant cell walls for lignin degradation, the role of various small organic/inorganic compounds, serving as redox mediators for lignin-degrading enzymes in lignin degradation, is discussed in detail.
机译：白腐真菌可以通过分泌的酶的作用选择性和有效地降解木质素。主要的木质素降解酶包括木质素过氧化物酶（LiP），锰过氧化物酶（MnP）和漆酶。 LiP和MnP需要过氧化氢（H_2O_2）才能发挥作用，但是它们会被高浓度的H_2O_2灭活。白腐真菌中有多种产生H_2O_2的酶。 LiP能够氧化酚醛和非酚醛木质素的亚结构，而MnP和漆酶只能降解酚醛木质素的亚结构。不饱和脂肪酸或含硫化合物可使MnP降解非酚类木质素亚结构。多项体外研究表明，某些酶可以协同降解木质素。由于木质素降解酶的体积太大，无法穿透植物细胞壁进行木质素降解，因此将详细讨论各种小的有机/无机化合物在木质素降解中作为木质素降解酶的氧化还原介体的作用。

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