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首页> 外文会议>22th Symposium on Biotechnology for Fuels and Chemical, 22th, May 7-11, 2000, Gatlinburg, Tennessee >Effect of a Natural Contaminant on Foam Fractionation of Bromelain
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Effect of a Natural Contaminant on Foam Fractionation of Bromelain

机译：天然污染物对菠萝蛋白酶泡沫分离的影响

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摘要

Foam fractionation is a simple, inexpensive method for separating and purifying proteins. Typically, a dilute bromelain solution with a pH ranging from 2.0 to 7.0 foams very well when bubbles are introduced into a foam fractionation column. It was observed, however, that the dilute enzyme solution only foamed between approximately pH 2.0 and 3.0 when the inner wall of the fractionation column was coated with a natural contaminant (okra residue). We studied the separation ratio and the protein mass recovery to explore the effect of a natural antifoaming agent on the foam fractionation of a dilute bromelain solution. The control variables used in this process were the initial bulk solution pH, which ranged from 2.0 to 7.0, and the superficial air velocity, which varied between 1.7 and 6.2 cm/s.
机译：泡沫分离是一种简单，廉价的分离和纯化蛋白质的方法。通常，当将气泡引入泡沫分馏塔中时，pH值为2.0至7.0的稀菠萝蛋白酶溶液会很好地发泡。然而，观察到，当分馏塔的内壁被天然污染物（秋葵残渣）覆盖时，稀酶溶液仅在约pH 2.0至3.0之间起泡。我们研究了分离率和蛋白质质量回收率，以探索天然消泡剂对稀菠萝蛋白酶溶液泡沫分离的影响。在此过程中使用的控制变量是初始本体溶液的pH值（范围从2.0到7.0）和表面空气速度（介于1.7和6.2 cm / s之间）。

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