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首页> 外文会议>Symposium on biotechnology for fuels and chemicals >Effect of a Natural Contaminant on Foam Fractionation of Bromelain
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Effect of a Natural Contaminant on Foam Fractionation of Bromelain

机译：天然污染物对菠萝蛋白酶泡沫分馏的影响

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Foam fractionation is a simple, inexpensive method for separating and purifying proteins. Typically, a dilute bromelain solution with a pH ranging from 2.0 to 7.0 foams very well when bubbles are introduced into a foam fractionation column. It was observed, however, that the dilute enzyme solution only foamed between approximately pH 2.0 and 3.0 when the inner wall of the fractionation column was coated with a natural contaminant (okra residue). We studied the separation ratio and the protein mass recovery to explore the effect of a natural antifoaming agent on the foam fractionation of a dilute bromelain solution. The control variables used in this process were the initial bulk solution pH, which ranged from 2.0 to 7.0, and the superficial air velocity, which varied between 1.7 and 6.2 cm/s.
机译：泡沫分馏是一种简单，廉价的分离和纯化蛋白质的方法。通常，当气泡引入泡沫分馏塔时，稀释溴溶液的溶液范围为2.0至7.0泡沫。然而，观察到稀酶溶液仅当分馏塔的内壁涂覆有天然污染物（秋葵残余物）时，稀酶溶液仅在大致pH 2.0和3.0之间发泡。我们研究了分离率和蛋白质质量回收，探讨了天然消泡剂对稀溴溶液的泡沫分馏的影响。在该过程中使用的控制变量是初始散粒溶液pH，其范围为2.0至7.0，浅表空气速度，其变化在1.7和6.2cm / s之间。

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