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首页> 外文会议>第四届国际板栗大会（Proceedings of Fourth International Chestnut Symposium) >Changes of Physiology and Biochemistry in Adventitious Root Formation of Castanea moilissima cv. 'Yanshanhong' in Vitro
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Changes of Physiology and Biochemistry in Adventitious Root Formation of Castanea moilissima cv. 'Yanshanhong' in Vitro

机译：板栗不定根形成的生理生化变化。燕山红体外

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摘要

To analyze the physiological and biochemical changes in adventitious root formation, endogenous hormone content and enzyme activity were measured. Shoot tips of C. mollissima were chosen in vitro to be rapidly micropropagated, inducted to root by two-step method. Shoots were treated with 3.0 mg·L-1 IBA in experimental group while some others without IBA in control group, and the samples were regularly collected and separately measured. The results were as follows: (1) During adventitious root formation, IAA content reduced slightly in the first 3 days, thereafter it rapidly climbed to the peak on the 7th day, and then reduced. ABA content kept steady and less than that of the control groups. CTK and GA3 content descended, and the content of CTK was significantly lower than that of the controls. The curve of the ratio of IAA/ABA was similar with IAA content's, while the ratio of IAA/CTK's curve showed no clear regularity. (2) The activities of IAAO and POD gradually increased in the first 3 days and then descended to minimum on the 7th day, on the contrary, the activity of PPO climbed and reached the peak on the 7th day. (3) The ratio of IAA/ABA and the activity of PPO were the key factors of adventitious root formation of C. mollissima.
机译：为了分析不定根形成的生理和生化变化，测量了内源激素含量和酶活性。在体外选择了毛梭菌的茎尖进行快速微繁殖，并通过两步法诱导其生根。实验组用3.0 mg·L-1 IBA处理枝条，对照组用无IBA处理枝条，定期收集样品并单独测量。结果如下：（1）在不定根形成过程中，IAA的含量在前3天略有下降，然后在第7天迅速攀升至峰值，然后下降。 ABA含量保持稳定并且低于对照组。 CTK和GA3含量下降，且CTK含量明显低于对照。 IAA / ABA的比值曲线与IAA含量的曲线相似，而IAA / CTK的比值曲线没有明确的规律性。 （2）IAAO和POD的活性在前3天逐渐增加，然后在第7天降至最低，相反，PPO的活性在第7天攀升并达到峰值。 （3）IAA / ABA的比值和PPO的活性是C. mollissima不定根形成的关键因素。

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