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首页> 外文期刊>Acta Horticulturae >Analysis of the Hormonal Manipulation during In Vitro Induction of Adventitious Roots in Castanea sativa
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Analysis of the Hormonal Manipulation during In Vitro Induction of Adventitious Roots in Castanea sativa

机译：板栗不定根离体诱导过程中的激素调控分析

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摘要

Adventitious rooting is a complex process still poorly understood, and is a crucial step for the clonal propagation of elite genotypes of woody species. The aim of this work was to develop a new, simple and reproducible experimental system in chestnutto study the adventitious rooting process at the physiological and molecular levels. Chestnut microshoots and leaf explants excised from microshoots were used to evaluate their physiological responses to indole-3-butyric acid alone or in combination with N-l-naphthyl-phthalamic acid. Similar rooting percentages were obtained in auxin treated microshoots and leaves, however, the mean number of roots per rooted explant was higher in microshoots than in leaves. When NPA was included in the medium, the rooting capacity of both, leaves and microshoots, was reduced and root emergence was delayed at least 4 days compared with explants treated with IBA alone. Whereas in microshoots less than half of the roots were developed from the basal callus, in leaf explants the majority of the roots were originated from the callus developed in the leaf petiole, which made this system very useful for the identification of auxin-responsive genes and key regulatory genes involved in the early events of adventitious rooting process.
机译：不定根是一个尚不为人所知的复杂过程，并且是木质物种优良基因型克隆繁殖的关键步骤。这项工作的目的是在栗子中开发一种新的，简单且可重现的实验系统，以从生理和分子水平研究不定根生根过程。从微枝中切下的栗微枝和叶片外植体用于评估它们对单独或与N-1-萘基-邻苯二甲酸的吲哚-3-丁酸的生理反应。在生长素处理的微枝和叶片中获得相似的生根百分比，但是，微枝中每个生根的外植体的平均根数高于叶片。与仅用IBA处理的外植体相比，当将NPA包括在培养基中时，叶子和微枝的生根能力均降低，并且根出苗至少延迟了4天。而在微枝中，只有不到一半的根由基部愈伤组织发育，而在叶外植体中，大多数根源于叶柄中发育的愈伤组织，这使得该系统对于植物生长素应答基因和与不定根生根过程的早期事件有关的关键调控基因。

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