首页> 外文会议>第四届国际板栗大会(Proceedings of Fourth International Chestnut Symposium) >Molecular Characterization of Hybrid Castanea Clones for Forest Reproductive Material Certification
【24h】

Molecular Characterization of Hybrid Castanea Clones for Forest Reproductive Material Certification

机译:用于森林繁殖材料认证的杂交栗树无性系的分子表征

获取原文
获取原文并翻译 | 示例

摘要

TRAGSA applied RAPDs methodology to molecular characterize the Castanea hybrids clone collection that had been on propagation in its own nursery for years. Nowadays, basic materials of all forest Galician nurseries are being tested using this same method for its approval or refusement. Three different 10-mer primers (number 2 and 4 from kit A and number 11 from kit S, Operon Technologies) are necessary to properly identify the whole collection. The system is repetitive, and shows all the clones are different and present their own DNA profile. Therefore, this method is useful for the molecular characterization and certification of the new hybrid Castanea clones included in the Spanish Catalogue. At the same time, four SSRs markers have been tested with the same purpose, due to their high reproducibility and discrimination power.
机译:TRAGSA应用RAPDs方法对已经在自己的苗圃中繁殖多年的Castanea杂种无性系进行了分子鉴定。如今,所有森林加利西亚苗圃的基本材料都使用相同的方法对其批准或拒绝进行测试。为了正确识别整个集合,必须使用三种不同的10-mer引物(试剂盒A的编号2和4,以及试剂盒S的编号11)。该系统是重复性的,显示所有克隆都是不同的,并显示出它们自己的DNA图谱。因此,该方法对于西班牙目录中包括的新杂种栗树无性系的分子鉴定和认证很有用。同时,由于具有很高的可重复性和区分能力,已经对四个SSR标记进行了相同目的的测试。

著录项

  • 来源
  • 会议地点 Beijing(CN)
  • 作者单位

    TRAGSA,Departamento de Mejora Agroforestal,Crta.Maceda-Valdrey,km.2,32700 Maceda,Ourense,Spain;

    TRAGSA,Departamento de Mejora Agroforestal,Crta.Maceda-Valdrey,km.2,32700 Maceda,Ourense,Spain;

    TRAGSA,Departamento de Mejora Agroforestal,Crta.Maceda-Valdrey,km.2,32700 Maceda,Ourense,Spain;

    Departamento de Biologia Vegetal y Ciencia del Suelo,Universidad de Vigo,Facultad de Biologia,36301 Vigo,Spain;

    Departamento de Fisiologia Vegetal,Universidad de Santiago de Compostela,Facultad de Biologia,15782 Santiago de Compostela,La Coruna,Spain;

    Departamento de Fisiologia Vegetal,Universidad de Santiago de Compostela,Facultad de Biologia,15782 Santiago de Compostela,La Coruna,Spain;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    RAPDs; SSRs; basic material; ten-mer primers; clonal identity;

    机译:RAPD; SSR;基本材料;十聚体引物;克隆身份;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号