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Molecular Characterization of Hybrid Castanea Clones for Forest Reproductive Material Certification

机译:杂交铸砂克隆对森林生殖材料认证的分子特征

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TRAGSA applied RAPDs methodology to molecular characterize the Castanea hybrids clone collection that had been on propagation in its own nursery for years. Nowadays, basic materials of all forest Galician nurseries are being tested using this same method for its approval or refusement. Three different 10-mer primers (number 2 and 4 from kit A and number 11 from kit S, Operon Technologies) are necessary to properly identify the whole collection. The system is repetitive, and shows all the clones are different and present their own DNA profile. Therefore, this method is useful for the molecular characterization and certification of the new hybrid Castanea clones included in the Spanish Catalogue. At the same time, four SSRs markers have been tested with the same purpose, due to their high reproducibility and discrimination power.
机译:Tragsa将RAPDS方法应用于分子表征Castanea Hybrids克隆收集多年来一直在自己的苗圃中繁殖。如今,所有森林加利西亚苗圃的基本材料都正在使用相同的方法进行批准或申请进行测试。需要三种不同的10-MEL引物(来自套件A和1号套件,操作典型技术的第2和第4页),以适当识别整个集合。该系统是重复的,并且显示所有克隆都不同并呈现它们自己的DNA谱。因此,该方法对于包括在西班牙目录中包括的新杂交铸砂克隆的分子表征和认证是有用的。同时,由于其高再现性和辨别力,四个SSRS标记已经以相同的目的进行了测试。

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