ISOLATION AND STRUCTURAL ANALYSIS OF A COLOURED COMPOUND FROM DISTILLERY SPENT WASH AND ITS BIOTRANSFORMED PRODUCTS

机译：酒糟洗涤液及其生物转化产物的有色化合物的分离与结构分析

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The studies on decolourisation of distillery effluent can not be carried out efficiently without the knowledge of the nature of colourant(s) and their properties. The presence of brown colour in distillery effluent is mainly due to the coloured compounds, which are formed during manufacturing of sugar from sugarcane juice. Caramels and melanoidins constitute the main colouring ingredients, which are formed by non-enzymatic reactions. In this study, one of the colouring compounds from the distillery effluent was purified by thin layer chromatography and adsorption chromatography. The structure of the purified coloured compound was elucidated by the application of the spectroscopic techniques, viz., UV, NMR, IR and Mass Spectoscopic analysis. The compound was characterized as 3, 4 dimethoxy 2 (1 hydroxy ethyl) α-naphthol. Biotransformation of the brown colouring compound was carried out by the treatment of predigested distillery effluent with indigenous bacterial consortia of activated sludge. The two colourless products formed after biotransformation were purified and characterized by UV, NMR, IR and MS analysis. The interpretation of the spectroscopic analysis led to the conclusion that the two products formed after biotransformation were 3, 4 dihydroxy α-tetralone (1) and 4 - hydroxy α-tetralone

机译：如果不了解着色剂的性质及其性质，就无法有效地进行蒸馏废水的脱色研究。酿酒厂废水中存在棕色的主要原因是有色化合物，这些化合物是在由甘蔗汁制糖过程中形成的。焦糖和黑色素是主要的着色成分，它们是通过非酶反应形成的。在这项研究中，通过薄层色谱法和吸附色谱法纯化了酒厂废水中的一种着色化合物。通过使用光谱技术，即UV，NMR，IR和质谱分析来阐明纯化的有色化合物的结构。该化合物的特征为3，4二甲氧基2（1羟乙基）α-萘酚。棕色化合物的生物转化是通过用活性污泥的原生细菌联合体处理预先消化的酒厂废水进行的。生物转化后形成的两种无色产物被纯化并通过UV，NMR，IR和MS分析表征。光谱分析的解释得出的结论是，生物转化后形成的两个产物分别是3，4二羟基α-四氢萘酮（1）和4-羟基α-四氢萘酮

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来源
《64th Annual Convention of the Sugar Technologists' Association of India Aug 17-19, 2002 Cochin (Kerala)》|2002年|p.C.8-C.30|共23页
会议地点 Cochin(IN);Cochin(IN)
作者
Moushumi Ghosh; Anil K. Tripathi; P. K. Agrawal;
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作者单位

Biochemistry Division, National Sugar Institute, Kanpur - 208017;

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会议组织
原文格式 PDF
正文语种 eng
中图分类轻工业、手工业;
关键词
spent wash; melanoidins; caramel; decolourisation; 3, 4 dimethoxy; 2(I hydroxy ethyl)α-naphthol; 3, 4 dihydraxy α-tetralone; 4 - hydroxy α-tetralone;

机译：用过的洗液;类黑素;焦糖;脱色; 3，4二甲氧基; 2（I羟乙基）α-萘酚; 3，4二羟基α-四氢萘酮; 4-羟基α-四氢萘酮;

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ISOLATION AND STRUCTURAL ANALYSIS OF A COLOURED COMPOUND FROM DISTILLERY SPENT WASH AND ITS BIOTRANSFORMED PRODUCTS

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