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首页> 外文会议>7th China-Japan International Congress of Microbiology Shanghai Symposium-2000 4-6 August 2000 Shanghai >Sequence analysis and PCR identification of Exophiala dermatitidis based on nuclear ribosomal-DNA sequence data
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Sequence analysis and PCR identification of Exophiala dermatitidis based on nuclear ribosomal-DNA sequence data

机译：基于核糖核糖体DNA序列数据的皮肤埃希氏菌序列分析和PCR鉴定

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摘要

Being neurophilic, Exophiala dermatitidis is the most virulent species in genus Exophila, which is a major causative agent of phaeohyphomycosis. The differentiation of Exophiala dermatitidis from other species, expecially Exophiala jeanselmei, according to phenotype only is very difficult, for it has no particular morphological characters. Based on partial nuclear ribosomal DNA sequence data, we have nalayzed strains of Exophiala dermatitidis from clinical specimens, and designed a species-specific primer targeted the conserved sequences of the internal transcribed spacer region. Polymerize strain reactions using this primer in conjunction with a universal backward primer were applied among more than sixty stock strains in our laboratory. Fifteen of Exophiala dermatitidis consist of standard strains of ATCC, CBS, IFM and BMU showed positive results, whereas all in the others included Exophiala jeanselmei, that was very closely related in phenotype to it, turned out to be negative. These data indicated that strains in Exophiala dermatitidis are homogeneous and therefore PCR serves to be a promising tool for the differentiation from other species, especially Exophiala jeanselmei. Together with phenotype method, geotype approach makes Exophiala identification perfectly.
机译：作为嗜神经的，皮肤软皮埃菲氏菌是Exophila属中最强毒的物种，它是疏生真菌的主要病原体。仅根据表型区分皮肤癣菌与其他物种，特别是Exophiala Jeanselmei是非常困难的，因为它没有特定的形态特征。基于部分核糖体DNA序列数据，我们从临床标本中分离了皮肤癣菌Exophiala dermatitidis的菌株，并设计了针对内部转录间隔区保守序列的物种特异性引物。使用该引物和通用反向引物的聚合菌株反应已在我们实验室的60多个原种菌株中应用。十五种由标准菌株ATCC，CBS，IFM和BMU组成的Exophiala dermatitidis均显示阳性结果，而其他所有菌株均包括Exophiala Jeanselmei，其表型与之密切相关，结果呈阴性。这些数据表明，皮肤癣菌中的菌株是同质的，因此PCR可以作为与其他物种，特别是牛仔裤色斑区别的有前途的工具。与表型方法一起，地型方法使Exophiala的识别更加完美。

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