...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of dermatological science >Rapid identification of Trichophyton tonsurans by specific PCR based on DNA sequences of nuclear ribosomal internal transcribed spacer (ITS) 1 region.
【24h】

Rapid identification of Trichophyton tonsurans by specific PCR based on DNA sequences of nuclear ribosomal internal transcribed spacer (ITS) 1 region.

机译：基于核糖体内部转录间隔区（ITS）1区域的DNA序列，通过特异性PCR快速鉴定扁毛癣菌。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

BACKGROUND: Trichophyton tonsurans, a dermatophyte implicated in an international epidemic of tinea capitis, was also found to be responsible for infecting wrestling and Judo athletes nationwide in Japan since 2001. OBJECTIVE: A rapid and highly accurate means of identifying this pathogen has been required to control the infection. We have developed a T. tonsurans-specific PCR method based on the DNA sequences of the nuclear ribosomal internal transcribed spacer 1 region. SUBJECTS: Eighteen species of six genera of standard strains and 75 strains of clinically isolated Trichophyton species were used in this study. METHODS: A T. tonsurans-specific PCR primer pair (tonsF1 and tonsR1) was designed on the nuclear ribosomal internal transcribed spacer 1 region, located between 18S and 5.8S rDNA. Fungal DNA was extracted from the colonies grown on culture plates, and the specificity of the PCR primers was tested. RESULTS: The specific PCR product was amplified from the standard strain of T. tonsurans and from five strains isolated from black dot ringworms, but there was no band from the 70 clinical isolates of other Trichophyton species. This T. tonsurans-specific PCR method was able to detect 10 pg of T. tonsurans genomic DNA with ethidium bromide staining. CONCLUSIONS: A PCR identification system specific for T. tonsurans is rapid, sensitive, and specific.
机译：背景：自2001年以来，在日本全国范围内，摔跤癣菌也被认为是导致摔跤和柔道运动员感染的一种原因。这是一种皮肤癣菌，与国际性的头癣流行有关。控制感染。我们已经基于核糖体内部转录间隔区1区域的DNA序列开发了一种扁桃体特异性PCR方法。研究对象：六个属的标准菌株的十八种和临床分离的毛癣菌物种的七十五种菌株。方法：在18S和5.8S rDNA之间的核糖体内部转录间隔区1区域上设计了扁桃体特异性PCR引物对（tonsF1和tonsR1）。从培养板上生长的菌落中提取真菌DNA，并测试PCR引物的特异性。结果：从扁桃体的标准菌株和从黑点癣中分离出的5种菌株中扩增了特异性PCR产物，但从其他毛癣菌属的70种临床分离株中未发现条带。该扁桃体特异性PCR方法能够通过溴化乙锭染色检测10 pg扁桃体基因组DNA。结论：针对扁桃体的PCR鉴定系统快速，灵敏且特异。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号