• 主题
高级搜索  >
历史搜索 清空历史
您现在的位置：首页> 中文期刊>医药卫生>大学学报(医药卫生)>广东药学院学报

广东药学院学报

广东药学院学报

CSTPCD

Journal of Guangdong Pharmaceutical University
本刊是广东药学院学报主办的自然科学期刊，主要反映本院及附属药厂、附属医院、教学单位、校友的科研成果，以及国内外最新医药科技信息。本刊为中国科技论文统计源期刊（中国科技核心期刊），同时为《中国核心期刊（遴选）数据库》、美国《化学文摘》（CA）、《中国期刊全文数据库》等数据库来源期刊。
  • 发文量：4427
  • 被引量：1335
  • H指数：1
  • 开始收录时间：-
  • CNKI综合因子：-
  • 维普期刊影响因子： 0.438
  • 万方期刊影响因子：-
  • 创刊时间：-
  • 国内刊号/CN：44-1413/R
    国际刊号/ISSN：1006-8783
  • 发行周期：双月刊
    邮发代号：-
  • 主管单位：广东省教育厅
    主办单位：广东药学院
广东药学院学报-联系信息
  • 主编：朱家勇
  • 电话：020-39352063
  • 邮箱：gdpu2599@163.net
  • 地址：广州市大学城外环东路280号
  • 邮编：510006
广东药学院学报-获奖情况
  • 暂无获奖信息
  • 收录汇总
  • 刊内文献检索
  • 投稿信息

刊期浏览

相关中文期刊

相关中文会议

相关外文期刊

相关外文会议

最新期刊

热门期刊

广东药学院学报 >2021年第6期

学科

年度

全选（0）
排序：
共4384条结果
  • 摘要：目前T细胞过继免疫治疗技术已应用于治疗各种疾病,尤其是在肿瘤领域的应用,具有肿瘤抗原特异性的T细胞过继免疫疗法不仪可以在体外实验中诱导肿瘤细胞死亡,而且已经部分应用于临床患者并达到一定的治疗效果,有望成为从基因水平上解决肿瘤疾病的前沿武器.本文对近年来T细胞过继免疫疗法在肿瘤疾病治疗方面的研究进行综述.%T cell adoptive immunotherapy has been employed to treat a variety of malignant diseases, especially tumors. Adoptive transfer of tumor-specific T cells induces the death of tumor cells in vitro, and has been applied to treat certain clinical patients and obtained successful therapeutic effects. Now,T cell adoptive therapy may become one of frontier weapons for treating tumors.
  • 摘要：Objective To determine the equilibrium solubility and the apparent oil/water partition coefficient of silymarin in different pH phosphate buffer solutions in 37 ℃. Methods A shake flask-ultraviolet spectrophotometry method was applied to determine the equilibrium solubility and partition coefficient of silymarin. Concentrations of silymarin in n-octanol-buffer solutions of different pH were detected by UV. Results The equilibrium solubility of silymarin in different phosphate buffer solution of pH 2.0,3.0,4.0,5.0, 6.0,6.8,7.4,8.0,9.0 were 0.673,0. 681,0.776,0.848,0.937,1.218,1.486,1.504,1.691 mg · mL-1, and the oil/water apparent partition coefficient of silymarin in different pH respectively were 57. 12,10. 83, 15. 56,7.39,10.19,3.82,2.75,1.79,0. 84 in 37℃. Conclusion The equilibrium solubility and apparent oil/ water partition coefficient were correlated to the pH of medium. With the increase of pH value, the equilibrium solubility rised, while the apparent oil/water partition coefficient decreased.%目的 考察水飞蓟素在37℃下,在不同pH磷酸盐缓冲溶液中的平衡溶解度和表观油水分配系数.方法 采用气浴恒温振荡器摇瓶法测定水飞蓟素的平衡溶解度及表观油水分配系数,紫外-可见分光光度法测定水飞蓟素在正辛醇-缓冲体系的质量浓度.结果 37℃时,水飞蓟素在pH 2.0、3.0、4.0、5.0、6.0、6.8、7.4、8.0、9.0的磷酸盐缓冲液中的平衡溶解度分别为0.673、0.681、0.776、0.848、0.937、1.218、1.486、1.504、1.691 mg· mL-1,相应条件下的表观油水分配系数分别为57.12、10.83、15.56、7.39、10.19、3.82、2.75、1.79、0.84.结论 水飞蓟素在磷酸盐缓冲溶液中的平衡溶解度及表观油水分配系数与介质的pH值相关,介质的pH值增大,水飞蓟素平衡溶解度增大,表观油水分配系数减小.
  • 摘要：Objective To prepare combined drug delivery system with 5-fluorouracil (5-FU) of chemically bonded with polyethyleneglycol-phosphatidyl (PEC-PE) ,and doxorubicin( DOX) of physical parcel in the hydrophobic nuclear of polymer complex micelles. Methods DOX loaded PEC-PE and S-fluorouracil-polyethyleneglycol-phosphatidyl ( 5-FU-PEC-PE ) were prepared by dialysis. Microscopic morphology, particle size and drug loading content were examined by means of TEM,NPA and UV respectively,and their drug release rates in vitro were determined. Results With 5-FU-PEG-PE as carrier, DOX loaded PEG-PE was prepared with loading content of 1.08% ,and the average particle size of 161.5 nm. The release of 5-FU from the micelles was regular without any burst effect. While the release of DOX from micelles included initiate with a burst release, then followed by good sustained release. Conclusion Drug/PEG-PE polymer micelles display perfect sustained and controlled release performance and can be potentially used as a new nano-pharmaceutial carrier.%目的 将化学键合在聚合物胶束聚乙二醇-磷脂酰乙醇胺(PEG-PE)的氟尿嘧啶(5-FU),与物理包裹于聚合物纳米胶束(PEG-PE)疏水核中的阿霉素(DOX)制备形成联合给药系统,并进行评价.方法 以5-FU-PEG-PE聚合物胶束为载体,采用透析法制备阿霉素(DOX)胶束；通过透射电镜、纳米粒度分析仪、紫外分光光度计等测定其形态、粒径、载药量,并考察其体外释放特性.结果 阿霉素(DOX)高聚物胶束载药量为1.08％,平均粒径为161.5 nm,聚合物胶束化学键合的5-FU释药速度稳定,无突释现象；物理包裹的DOX释放过程包括突释和缓释,表现出较好的缓释效果.结论 PEG-PE载药胶束是一种缓释性能良好、有应用前景的药物载体.
  • 摘要：目的 研制溴吡斯的明(PB)的阳离子脂质体,并对其体外释药进行考察.方法 采用逆向蒸发法制备PB脂质体(PB-L),以N-三甲基壳聚糖(TMC60)为包衣材料对其进行包衣,制备阳离子型PB-L,并用正交设计法对其处方进行优化；对溴吡斯的明脂质体包衣前后的形态、粒径、Zeta电位及体外释药特性进行考察.结果 最优处方为卵磷脂∶胆固醇为4∶1(质量比),药物∶类脂为1∶25(质量比),TMC60溶液质量浓度为0.4 mg · mL-1,磷酸盐缓冲液的pH为7.4.脂质体外形圆整,粒径在包衣后略有增加,Zeta电位由负转正,平均包封率为(63.36±0.27)％(n=3),体外释药符合Higuchi方程:Q=-0.2015+0.3252t1/2,r=0.989 9,具有明显的缓释特性.结论 TMC60包衣PB-L带正电荷,包封率较高,值得进一步开展体内研究.%Objective To prepare cationic liposomes loaded pyridostigmine bromide (PB) and study their in vitro release. Methods The PB liposomes ( PB-L) were prepared by reverse phase evaporation followed by N-trimethyl chitosan (TMC60) coating. Orthogonal design was used to optimize the TMC60-coated PB-L formulation. The morphology, size, Zeta potential and in vitro release property of the formulation were studied compared with the uncoated PB-L. Results The optimal technological conditions were as the follows: the ratio of lecithin and cholesterol of 4:1(ζ) ,the ratio of PB and lipids of 1 : 25(ζ) ,the TMC60 concentration of 0. 4 mg · mL-1 and PBS with pH of 7. 4. After TMC60 coating, the PB-L were with spherical morphology, slightly increased size, positive Zeta potential and average entrapment efficiency of (63.36 ±0. 27)% (re = 3). The in vitro release property was accorded with Higuchi equation; Q = -0. 2015 + 0. 3252t1/2 , r = 0. 989 9, showing significant sustained release property. Conclusion TMC60-coated PB-L with positive charge and high entrapment efficiency is valuable to be further studied in vivo.
  • 摘要：Objective To investigate the modulation of Prunella vulgaris L. (PPV) polysaccharide on immunosuppressive mice induced by cyclophosphamide (CTX). Methods Mice were injected with CTX to establish the immunosuppressive model. The effects of different doses of PPV on the index of thymus and spleen, peritoneal macrophage activity and the levels of blood serum hemolysin and hemolytic plaque in mice were detected. Results PPV was effective in improving the immune function of immunosuppressive mice. Compared with the control group, PPV treatment significantly improved the index of thymus and spleen , increased the phagocytic activity of peritoneal macrophage, and improved the formation of hemolysin and hemolytic plaque in immunosuppressive mice. Conclusion PPV could enhance the immunological activity in immunocompromise mice.%目的 研究夏枯草多糖对环磷酰胺所致免疫低下模型小鼠免疫功能的调节作用.方法 采用腹腔注射环磷酰胺(cyclophosphamide,CTX)复制免疫功能低下小鼠模型,并考察不同剂量夏枯草多糖对免疫抑制小鼠胸腺指数、脾脏指数、腹腔吞噬细胞吞噬功能、血清溶血素生成及溶血空斑形成的影响.结果 夏枯草多糖中、高剂量组能显著提高免疫功能低下小鼠的脏器指数,增强腹腔巨噬细胞吞噬功能,促进溶血素水平及溶血空斑的形成.结论 夏枯草多糖具有良好的免疫增强活性.
  • 摘要：目的 应用分子生物学方法鉴定粘质沙雷氏菌和红曲霉原生质体跨界融合子.方法 采用随机扩增多态性分析和功能基因PCR扩增的方法对9个融合子进行分子鉴定.结果 筛选出的6条随机引物对2个亲本和9个融合子的DNA均能扩增出清晰的条带,除第5号融合子和粘质沙雷氏菌的相似指数略低,其余8个融合子和2个亲本的相似指数均大于2个亲本之间的相似指数.红曲霉和9个融合子中均能扩增出红曲霉功能基因mKH的条带,从粘质沙雷氏菌中则未扩增出相应条带.结论 用分子生物学方法证实获得的9个能产红色素的菌株均为粘质沙雷氏菌和红曲霉的跨界融合子.%Objective To identify the inter-kingdom fusants of Senatia marcescens and Monascus with molecular biology method. Methods The random amplified polymorphic DNA ( RAPD) and functional gene amplification assay were employed to identify the nine fusants. Results Distinct DNA fragments were obtained with the screened six random primers from both parent strains and nine fusants. Except the legs similarity between the No. 5 fusant and Serratia marcescens, the similarity between the eight fusants and two parent strains was greater than that between the two parent strains. The functional mkH gene of Monascus can be amplified from the nine fusants and Monascus rubber, but not detected from the DNA of Serratia marcescens. Conclusion The nine pigmented fusants were successfully identified to be the inter-kingdom fusants from Serratia marcescens and Monascus.
  • 摘要：目的 设计合成2个手性钌(Ⅱ)多吡啶配合物Λ-[Ru(bpy)2(P-NPIP)](PF)2·2H2O(1)和Λ-[Ru(bpy)2(p-tFPIP)](PF6)2·2H2O(2),评价其体外抗肿瘤活性,并对配合物2与DNA分子的识别机制及其光裂解作用进行初步探讨.方法 采用MTT方法评价手性钌(Ⅱ)配合物1和2对肿瘤细胞生长的抑制,并采用电子吸收光谱、荧光光谱和黏度试验等方法研究手性钌配合物2与DNA的分子识别机制；采用凝胶电泳试验评价配合物2对DNA分子的光裂解作用.结果 手性钌(Ⅱ)配合物2能够抑制肿瘤细胞的生长,特别是对HO8910PM细胞生长的抑制率IC50=47.8 μmol·L-1,与同等条件下顺铂的抗肿瘤活性相当；研究结果表明插入配体上取代基的电子效应对其抗肿瘤活性有较大影响；光谱学试验证明钌多吡啶配合物1和2通过插入方式与DNA结合(Kb =2.86×105 L· mol-1).结论 手性钌(Ⅱ)多吡啶配合物具有一定的抗肿瘤活性,其抗肿瘤活性与钌(Ⅱ)配合物的分子结构及其与DNA分子契合能力相关.%Objective To synthesize and characterize two chiral ruthenium( Ⅱ ) complexes A-[ Ru(bpy)2 (p-NPIP)](PF6)2 ·2H2O (1) andA-[Ru(bpy)2(p-tFPIP)](PF6)2 · 2H2O (2) by ES-MS/H-NMR and CD spectra,and investigate the antitumor activity of both complexes and its interaction with DNA. Methods The antitumor activity of the two complexes against tumor cells were evaluated by MTT methods, and the interaction of chiral ruthenium ( Ⅱ ) complex 2 with calf thymus DNA has been investigated by electronic absorption spectra and fluorescent spectra,as well as viscosity measurements. Results The results show that complex 2 exhibits excellent inhibitory activity against HO8910PM cell with IC50 =47. 8 μmol · L-1,which is comparable to that of cis-platinum at the same conditions,and the chiral ruthenium complex binds to DNA through intercalation mode with binding constant measured with 2. 86 × 105 L · mol-1. Conclusion The chiral ruthenium ( Ⅱ) complexes exhibit promising anti-tumor activity against varied tumor cells,which involves the binding properties of ruthenium(Ⅱ ) complexes with DNA molecules.
  • 摘要：目的 建立五指柑药材中木犀草素及总黄酮的含量测定方法.方法 采用HPLC法测定木犀草素含量,色谱柱:Kromasil C18色谱柱(250 mm×4.6 mm,5μm)；流动相:乙腈-0.03％(体积分数)H3 PO4(体积比25∶75)；流速:1.0 mL/min；检测波长:350 nm.总黄酮测定采用Al(NO3)3显色法.结果 木犀草素在0.184～1.564 μg范围内与峰面积呈良好线性关系(r=0.999 1),精密度良好,平均回收率99.22％.测定了3批五指柑药材中木犀草素及总黄酮的质量分数,两者分别为0.364、10.96 mg/g.结论 建立的木犀草素及总黄酮含量测定方法准确可行、重复性好,可用于五指柑药材的质量评价.%Objective To determine the contents of luteolin and total flavonoids from Folium Vitex negundo. Methods HPLC was performed on kromasil C18 (250 mm ×4. 6 mm,5 μm) column with the mobile phase of acetonitrile-0.03%H3PO4(25:75) at the flow rate of 1.0 mL/min,and the UV detector was set at 350 nm. Total flavonoids were determined by Aluminum nitrate chromogenic method. Results The calibration curve was linear in the range of 0. 184 - 1. 564 μg( r = 0. 999 1 ) , and the average recovery ratio was 99. 22%. The average contents of luteolin and total flavonoids from Folium Vitex negundo were 0. 364 and 10. 96 mg/g. Conclusion The determination methods were simple,rapid and reproducible,and suitable for the quality control of Folium Vitex negundo.
  • 摘要：目的 建立胃灵冲剂中芍药苷和甘草苷的含量测定方法.方法 采用HPLC法,色谱柱为Hypersil ODS2(5μm,4.6 mm ×200 mm)柱,流动相为乙腈-0.1％(体积分数)磷酸溶液(体积比14∶86),检测波长为237 nm,流速为1.0 mL·min-1.结果 芍药苷的线性范围为0.1140 ～0.5700 μg(r =0.999 3),平均回收率为98.6％(n=6),RSD为0.7％；甘草苷的线性范围为0.211 4～1.057 0μg(r=0.999 5),平均回收率为98.2％(n=6),RSD为1.1％.结论 该方法简便准确、重复性好,可用于该制剂的质量控制.%Objective To establish a method for the determination of paeoniflorin and licorice glycoside in Weiling granules by HPLC. Methods The determination was carried out on a Hypereil ODS2(5 μm,4. 6 mm × 200 mm) column with a mobile phase of acetonitrile - 0. 1 % phosphoric acid solution (14: 86 ). The detective wavelength was set at 237 nm and the flow rate was 1. 0 mL · min -1. Results The linear range of paeoniflorin and licorice were 0. 114 0 - 0. 570 0 μg( r = 0. 999 3 ) and 0. 211 4 - 1. 057 0 μg( r = 0. 999 5). The average recovery rations of paeoniflorin and licorice were 98. 6% and 98. 2% ,RSD were 0.7% and 1. 1%. Conclusion This method is simple, accurate and reproducible, suitable for quality control of the drug.
  • 摘要：目的 探讨女性绝经后乳腺癌术后放疗和芳香化酶抑制剂(aromatase inhibitors,AI)内分泌治疗的时序性.方法 收集2002年1月～2007年12月收治156例女性绝经后激素受体阳性的乳腺癌患者资料,分为两组,77例放疗同期联合AI内分泌治疗(A组)；79例放疗后序贯AI内分泌治疗(B组).随访统计两组毒副反应发生率及长期生存率.结果 A组中位随访时间58月,B组中位随访时间55月；A组和B组放射性肺炎、放射性肺纤维化、放射性皮肤纤维化发生率分别为6.7％与6.3％(P=1.0)、12％与5.1％(P=0.122)、8％与3.8％(P=0.319)；A组和B组的复发率、远处转移率分别为36％与38％(P =0.800),40％与35.4％(P =0.560)；A组和B组5年总生存率、无复发生存率、无远处转移生存率分别为74.8％与73.8％(P =0.607)、73.8％与75.1％(P =0.577)、70.8％与71.2％(P =0.683)；多因素分析中乳腺癌术后放疗联合AI内分泌治疗的时序性不是预后的独立因素.结论 女性绝经后乳腺癌术后无论序贯或同期联用放射治疗和AI内分泌治疗都是安全可行的,但仍需进行前瞻性研究.%Objective To investigate the optimal sequence of adjuvant radiotherapy and hormonal therapy with aromatase inhibitors (AI) in post-menopause breast cancer patients after operation. Methods 154 female breast cancer patients with post-operation, post-menopause and hormone receptor positive were eligible for the retrospective study. Some patients were classified as A group ( n = 77) in which hormonal therapy with AI combined concurrently with radiotherapy, and other patients belonged to B group ( n - 79) in which hormonal therapy with AI were followed by radiotherapy. Treatment complications and prognostic outcomes were followed up and analyzed. Results The median follow-up was 58 months in A group and 55 months in B group. The occurrence rate of radiation pneumonitis,radiation fibrosis of lung and skin in both A and B groups were 6.7% vs 6.3% ,12% vs 5.1% and 8% vs 3. 8% Respectively. The local relapse and distant metastasis rates in two groups were 36% vs 38% and 40% vs 35.4%. The 5-year overall survival rate,relapse-free survival rate and metastasis-free survival rate in two groups were 4.8% vs 73. 8% ,73. 8% vs 75.1 % and 70. 8% vs 71.2% ,respectively. The multivariate analysis revealed that the sequence of radiotherapy and hormonal therapy with AI in post-operation breast cancer patients was not the independent prognostic factor for overall survival rate. Conclusion The sequence of radiotherapy and hormonal therapy with AI in post-menopause breast cancer patients after operation dose not affect long term survival.
  • 摘要：Enaminones are widely utilized as important intermediate for organic synthesis and have gained growing attention. This paper briefly reviews the advances in the synthesis of enaminones, especially the new methods to prepare enaminones including condensation reaction,cleavage of heterocycle and addition reaction. The advantages and disadvantages of these synthetic methods are discussed.%烯胺酮类化合物是重要的有机合成中间体,在现代科学领域中有广泛的应用.近年来其合成受到人们的广泛关注.本文总结了烯胺酮类化合物国内外的合成进展,介绍了烯胺酮的合成方法,如缩合、加成、杂环分解,并对其合成方法的优缺点进行讨论.
  • 摘要：麦冬为百合科植物,以块根入药,有滋阴生津、润肺止咳、清心除烦的功效,是我国大宗常用药材.本文综述麦冬植物资源状况以及主流商品种类的植物学特征和品种鉴别,有助于麦冬资源利用和深入研究.%Ophiopogon japonicus, which is a member of the Liliaceae family, is the cardinal herb indicated for Yin deficiency,hacking dry coughs, dry tongue and mouth. Research advances in the Cermplasm resources of Ophiopogon japonicus are reviewed, including herbalogical studies, classification and identification.
  • 摘要：Objective To investigate the chemical constituents of volatile oil from Eucalyptus urophylla S. T. Blakely leaves collected in Guangdong Province of China. Methods The volatile oil was extracted with steam distillation method,and analyzed by gas chromatography-mass spectrometry (GC-MS) method. The relative amount of each constituent was calculated by peak area normalization. Results Thirty-eight constituents in volatile oil from Eucalyptus urophylla S. T. Blakely leaves in Guangdong Province were identified,amounting to 97. 76% of the total area of the peaks. The principal constituents were eucalyptol (38.13% ) ,alpha-pinene(10.71% ) .menthol (9. 79% ) ,alpha-terpineol (7.41% ) ,neomenthol(5. 51% ), (R)-citronellol(5. 22% ) , alpha-terpinyl acetate (5.03% ) and so on. Conclusion The constituents of the volatile oil from Eucalyptus wophyllaS. T. Blakely leaves in Guangdong Province were successfully identified. Compared with the reports, our findings suggest great similarity and little differences, which provides the foundation for further comprehensive utilization of the volatile oil.%目的 研究广东尾叶桉叶挥发油的化学成分.方法 采用水蒸气蒸馏法提取广东尾叶桉叶挥发油,用气相色谱-质谱联用技术进行分离测定,结合计算机检索技术对分离的化合物进行结构鉴定,应用气相色谱峰面积归一化法确定各组分的相对含量.结果 首次确定了广东尾叶桉叶挥发油中38个化合物,所鉴定成分占总馏出峰面积的97.76％.主要成分为1,8-桉叶素(38.13％)、α-蒎烯(10.71％)、薄荷醇(9.79％)、α-松油醇(7.41％)、异蒲勒醇(5.51％)、右旋香茅醇(5.22％)、乙酸松香酯(5.03％)等.结论 确定了广东尾叶桉叶挥发油的化学成分,与文献报道尾叶桉挥发油的成分具有相似性,但仍存在一些差异,为综合利用桉叶挥发油奠定了基础.
  • 摘要：目的 研究苦参化学成分.方法 采用柱层析色谱方法对苦参进行分离纯化,根据理化性质和波谱数据鉴定其结构.结果 从苦参中分离鉴定了8个化合物,分别为三叶豆紫檀苷(1)、高丽怀素(2)、4-羟基-3-甲氧基-8,9-二亚甲基二氧紫檀素(3)、异去氢淫羊藿素(4)、降脱水淫羊藿素(5)、7,4’-二羟基异黄酮(6)、7-甲氧基-4’-羟基异黄酮(7)、7-甲氧基香豆素(8).结论 化合物7、8为首次从该植物中分离得到,并对化合物4已有报道的碳谱数据进行了补充.%Objective To investigate chemical constituents from Sophora flavesceru. Methods Compounds were isolated and purified by various chromatographic techniques. And their structures were elucidated with physicochemical properties and spectroscopic data. Results Eight chemical constituents were isolated and identified; trifolirhizin(1) ,maackiain(2),( - )-4-Hydroxy-3-methoxy-8,9-methyl-lenedioxypterocarpan(3), isoanhydroicaritin( 4 ) , noranhydroicaritin ( 5 ) , daidzein ( 6), 7-methoxy-4'- hydroxyisoflavone (7), 7-methoxy coumarin(8),respectively. Conclusion Compound 7 and 8 are isolated from this plant for the first time, and 13C-NMR spectra data of compound 4 were supplemented.
  • 摘要：Objective To establish a HPLC-ELSD method for the determination of diclofenac sodium in self-emulsifying sustained-release tablets. Methods HPLC was performed on the column of TC-C18(4. 6 mm × 250 mm,5 μm). The mobile phase was methanol-0.12% glacial acetic acid (20: 80) ,and the flow rate was 1.0 mL · min-1 . Drift tube temperature was 40 ℃ , the gas flow was 2.4 L · min -1 , and the gain was 1. The injection volume was 20 μL. Results The calibration curve of diclofenac sodium was linear in the range of 0. 295 -4.13 μg, r =0. 999 8. The average recovery ratio was 100. 34% with the RSD value of 2.41% (n=6) . The average content of diclofenac sodium in self-emulsifying sustained-release tablets was 25. 30 mg in each tablet. Conclusion The method can be used for the determination of diclofenac sodium in self-emulsifying sustained-release tablets.%目的 建立高效液相色谱-蒸发光散射检测法测定双氯芬酸钠自乳化缓释片中双氯芬酸钠的含量.方法 采用HPLC-ELSD法.色谱柱:Agilent TC-C18(250 mm×4.6 mm,5 μm)柱；流动相:甲醇-0.12％(体积分数)冰醋酸(体积比20∶80)；流速:1.0 mL· min-1；漂移管温度:40℃,气体流速:2.4 L·min-1,增益:1；进样量:20 μL.结果 双氯芬酸钠进样量在0.295 ～4.13 μg范围内,其自然对数值与峰面积积分值的自然对数值呈良好线性关系(r=0.999 8),加样回收率为100.34％(RSD =2.41％).6批双氯芬酸钠自乳化缓释片中双氯芬酸钠平均质量分数为25.30 mg·片-1.结论本方法快速、灵敏度高、重现性好,能快速准确测定自乳化缓释制剂中双氯芬酸钠的含量.
  • 摘要：Objective To evaluate the correlation of CYPlAl gene Msp I polymorphism and CYP2D6 * 10 allele C188T polymorphism with the genetic susceptibility to lung cancer. Methods The distribution frequencies of CYPlAl and CYP2D6 gene polymorphisms were detected in 86 lung cancer cases and 86 controls by polymerase chain reaction-restriction fragment length polymorphism ( PCR-RFLP) , and the relationship between the genetic polymorphism and genetic susceptibility to lung cancer was also analyzed. Results The frequencies of CYPlAl gene CC,TC and TT genotypes in case group were 17.44% ,38. 37% and 44. 19% , but that were 12. 79% , 29. 07% and 58. 14% in control group. Compared with TT genotype, the odd ratios of TT and TC genotypes were 1.79 (OR95%C/:0. 68 -4. 77) and 1. 74 (OR95% CI:0. 84-3.59). The frequencies of CYP2D6 gene C188/C,C188/T and T188/T genotypes in case group were 31.40% ,38. 37% and 30. 23% , but that were 44. 19% ,33. 72% and 22. 09% in control group. Compared with T188/T genotype ,the odd ratios of C188/C and C188/T genotypes were 1. 93 ( OR95% CI:0. 83 - 4. 48 ) and 1. 60 ( OR95% C/:0. 75 - 3. 44). The individuals with both CYP1AI (CC + TC) genotypes and CYP2D6 ( C188/C + C188/T) genotypes showed 2. 75-fold increase of risk ( OR95% CI: 1.06-7. 20). Conclusion Single polymorphism of CYPlAl and CYP2D6 does not show the risk of developing lung cancer, but the combination of two genetic polymorphisms significantly increases the risk of developing lung cancer.%目的 探讨代谢酶CYP1A1基因MspⅠ位点多态性、CYP2D6*10等位基因C188T位点多态性与人群肺癌遗传易感性之间的相关性.方法 应用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)技术检测86例广州市肺癌患者和86例对照的CYP1A1、CYP2D6基因多态性分布频率,分析基因多态性与肺癌遗传易感性之间的相关性.结果 CYP1A1基因CC、TC、TT在病例组的携带率分别为17.44％、38.37％、44.19％,对照组分别为12.79％、29.07％、58.14％.和TT组比较,CC和TC组OR值为1.79(OR95％ CI:0.68 ～4.77)和1.74(OR95％ CI:0.84 ～3.59)；CYP2D6基因C188/C、C188/T、T188/T病例组的携带率分别为31.40％、38.37％和30.23％,对照组分别为44.19％、33.72％和22.09％,和T188/T组比较,C188/C、C188/T OR值为1.93(OR95％ CI:0.83～4.48)、1.60(OR95％CI:0.75～3.44)；CYP2D6与CYP1A1联合作用时,CYP1A1(CC+TC)型基因及CYP2D6(C188/C+C188/T)型基因者患肺癌的风险为2.75(1.06～7.20).结论 CYP1A1、CYP2D6两种基因单独存在时未显示出与肺癌风险的联系,两种基因多态的联合作用明显提高了肺癌的危险性.
  • 摘要：Objective To investigate the chemical constituents from flex asprella. Methods Compounds were isolated by repeated column chromatographies with silica gel, and the structure was elucidated by spectral analysis and chemical evidence. Results Six compounds were isolated and their structures were identified as protocatechuic acid( Ⅰ ) , β- sitosterol( Ⅱ ) ,coniferyl aldehyde( Ⅲ) ,ilexolic acid( Ⅳ) ,daucosterol( Ⅴ )and 3-O-a-L-arabinopyranosyl pomolic acid ( Ⅵ). Conclusion Compounds Ⅲ , Ⅲ and VI were isolated from Ilex asprella for the first time.%目的 对岗梅根的化学成分进行研究.方法 采用多种柱层析方法进行分离纯化,并根据理化性质和波谱数据鉴定其结构.结果 从岗梅根的乙酸乙酯部位和正丁醇部位分离得到6个化合物,分别鉴定为原儿茶酸(Ⅰ)、β-谷甾醇(Ⅱ)、松柏醛(Ⅲ)、毛冬青酸(Ⅳ)、胡萝卜苷(Ⅴ)和3-O-α-L-阿拉伯吡喃糖基-19α-羟基-乌苏酸(Ⅵ).结论 化合物Ⅰ、Ⅲ和Ⅵ为首次从该植物中分离得到.
  • 摘要：目的 对多枝雾水葛进行生药学鉴定.方法 利用性状鉴别、显微鉴别和理化鉴别方法对多枝雾水葛进行生药鉴定研究.结果 粉末中草酸钙簇晶众多,非腺毛多为单细胞,表面光滑或有疣状突起.结论 确定了多枝雾水葛的性状、显微特征,可为多枝雾水葛的生药鉴定提供参考.%Objective To establish methods for the pharmacognostical identification of Pouzolzia zeyianica. Methods The plant was identified by characteristic study, microscopic identification, physical and chemical identification methods. Results Massive calcium oxalate crystals were observed in the plant powder. Non glandular hairs were single cells with smooth surface or wart. Conclusion The characteristic and microscopic results are confirmed,which can be taken as the reference for the pharmacognosy identification of the plant.
  • 摘要：Objective To establish the quality specification for Yangxue Qingnao Granules. Methods Angelicae Sinensis Radix, Chuanxiong Rhizoma, Spatholobi Caulis, Pulsatillae Radix, Prunellae Spica, Cassiae Semen and Rehmanniae Radix Praeparata were identified by TLC. Ferulic acid and paeoniflorin were determined by HPLC. Results Angelicae Sinensis Radix,Chuanxiong Rhizoma,Spatholobi Caulis,Pulsatillae Radix, Prunellae Spica,Cassiae Semen and Rehmanniae Radix Praeparata could be identified by TLC. Paeoniflorin and ferulic acid were linear at the range of 8.0-120.0 μg/mL(r =0. 999 9,n =6) and 1.0-12.0 ng/mL(r =0. 999 9, n =6) .respectively. The average recovery rates of ferulic acid and paeoniflorin were 95.64% (RSD =0. 51% , n=6) and 101. 53% (RSD = 0. 73% , n =6). Conclusion These methods are accurate, exclusive and reproducible,which may be suitable for the quality control of Yangxue Qingnao granules.%目的 建立养血清脑颗粒的质量标准.方法 采用薄层色谱法鉴别养血清脑颗粒中的当归、川芎、鸡血藤、夏枯草、白芍、决明子、熟地黄；采用高效液相色谱法测定养血清脑颗粒中芍药苷、阿魏酸的含量.结果 在薄层色谱中检出了当归、川芎、鸡血藤、夏枯草、白芍、决明子、熟地黄的特征斑点；高效液相色谱法测出芍药苷和阿魏酸分别在8.0～120.0 μg/mL、1.0～12.0μg/mL范围内与峰面积呈良好的线性关系,r分别为0.999 9(n =6)、0.999 9(n =6),平均回收率分别为95.64％、101.53％,RSD分别为0.51％(n=6)、0.73％(n=6).结论 本方法简单、专属性强,可作为养血清脑颗粒的质量控制方法.
  • 摘要：Objective To develop a capillary gas chromatography method for the determination of 4 kinds of residual organic solvents in cinepazide maleate. Methods Residual organic solvents ( cyclohexane, dichlormethane.ethonal and methyl benzene) in cinepazide maleate were separated by PEG-20M capillary column (30 m × 0. 25 mm × 0. 25 μm) with a FID detector. The injector temperature and the detector temperature were controlled at 200 ℃ and 250 ℃, respectively. The column temperature program was as follows; initial temperature 40 ℃ (hold for 6 min) ,with the increasing rate of 10 ℃/min to 60 ℃ (hold for 1 min) ,50 ℃/min to 220 ℃ ( hold for 2 min). The assay of residual solvents was calculated by internal standard method. The internal standard substance was methanol. Results The residual solvents ( cyclohexane, dichlormethane.ethonal and methyl benzene) were separated completely. The calibration curve of each solvent showed good linear relationship. The average recovery ratios were between 97.4% -103. 8%. Conclusion The method is simple,accurate and reliable,which can be used for the determination of the residual organic solvents in cinepazide maleate.%目的 建立马来酸桂哌齐特中4种有机残留溶剂的测定方法.方法 采用毛细管气相色谱法对马来酸桂哌齐特中环己烷、二氯甲烷、乙醇和甲苯的残留量进行测定.色谱柱:PEG-20M毛细管(30 m×0.25 mm ×0.25 μm)柱；进样口温度:200℃,FID检测器温度:250℃,初始柱温为40℃,保持6 min,以10℃/min升到60℃,保持1 min,再以50℃/min升到220℃,保持2min；载气为氮气,甲醇为内标物.结果 环己烷、二氯甲烷、乙醇和甲苯均能得到有效分离,其峰面积与质量浓度均呈良好的线性关系,平均回收率为97.4％ ～ 103.8％.结论 该方法简便、准确、灵敏度高,可用于马来酸桂哌齐特中有机残留溶剂的检测.
  • 摘要：Objective To analyze the clinical manifestations of adverse drug reactions (ADR) of intravenous gatifloxacin, to provide guidance for clinical drug safety measures. Methods The 566 cases of ADR of gatifloxacin intravenous infusion that occurred in a hospital from June 2009 to September 2010 were reported and analyzed. Results Among the 566 cases of ADR reports,the age of patients ranged from 16 to 89 years,with an average age of 37.51 years; 247 cases were male,319 female, and the male to female ratio was 1:1.29, the incidence of male ADR was slightly lower than female. The most common ADRs were skin symptoms (34. 04% ), gastrointestinal symptoms (21. 13%) and local symptoms at the sites of intravenous administration (17.14%). Conclusion; Problems existed in the intravenous administration of gatifloxacin in the hospital were mainly super indications, high infusion rates. The majority of ADRs can be avoided through better efforts of the reinforcement of rational use of drugs.%目的 分析静脉滴注加替沙星不良反应的临床表现,为临床安全用药提供指导.方法 对我院2009年6月～2010年9月发生的566例静脉滴注加替沙星导致的药品不良反应(adverse drug reaction,ADR)报告进行统计分析.结果 566例ADR报告中,年龄16～89岁,平均年龄37.51岁.男247例,女319例,男女比例1∶1.29,男性ADR发生率略低于女性.ADR主要表现为皮肤症状(34.04％)、胃肠道症状(21.13％)以及静脉给药局部症状(17.14％).结论 我院注射用加替沙星存在超适应证、滴注速度偏快等现象,绝大多数ADR可通过合理用药予以避免,应加大对注射用加替沙星合理用药的干预力度.
  • 摘要：Objective Predicting the antigen characteristics of short transient receptor potential channel of protein6 ( TRPC6) and screening appropriate synthetic polypeptide of TRPC6, in order to prepare the specific anti-TRPC6 monoclonal antibody. Methods Analysis of TRPC6 amino acid sequence by IEDB and Lasergene710WinInstall software,and prediction of epitope,antigenicity,hydrophilia and secondary structure of TRPC6. Results The synthetic polypeptide sequence was determined,which is consisted of 565-579 amino acid of TRPC6 extracellular domain. The surface accessibility, flexibility, beta-turn, antigenicity and hydrophilicity were 1.000,1. 008,1. 022,1. 034 and 2. 043, respectively. Conclusion The prediction of TRPC6 synthetic polypeptide meets the requirement for preparing anti-TRPC6 monoclonal antibody.%目的 预测瞬时受体电位阳离子通道蛋白6(TRPC6)的抗原表位特性,筛选合适的TRPC6氨基酸序列合成多肽,用于制备抗TRPC6合成肽单克隆抗体.方法 根据TRPC6氨基酸序列,采用IEDB分析及蛋白质软件(Lasergene710 WinInstall)分析,预测TRPC6表面性、抗原性、亲水性及可能的二级结构性质,以此筛选合成多肽序列.结果 确定合成多肽为TRPC6的胞外段565-579氨基酸序列,其表露性预 测值1.000；柔韧性预测值1.008；β-转角预测值1.022;抗原性预测1.034；亲水性预测2.043.结论 所确定的TRPC6多肽序列表位预测符合要求,为TRPC6合成肽制备抗单克隆抗体提供了一条有效的途径.
  • 摘要：Objective To investigate the effect of Gynura procumbent ( Lour. ) MERR. Extract ( GPE) on proliferation and cell cycle in U2-OS cells. Methods MTT assays were performed to study the inhibition of GPE on the growth of U2-OS cells,based on which IC50 was determined. The effect of GPE on the apoptosis of U2-OS cells was tested using Annexin V-FITC assay kit,and changes of cell cycle were detected by Flow cytometry. Results GPE inhibited the growth of U2-OS cells in vitro in a dose-dependent manner,with 1C50 value of 72 μg · mL-1. GPE was testified to be able to induce apoptosis in U2-OS cells. And U2-OS cells was found by Flow cytometry to be arrested in G0/G1 phase. Conclusion Gynura procumbent ( Lour. ) MERR. May be a potential antitumor herb.%目的 研究蛇接骨草提取物对U2-OS细胞的增殖抑制作用及对U2-OS细胞周期的影响.方法 以 95％乙醇进行提取(提取物简称GPE).MTT法检测GPE对U2-OS细胞的增殖抑制作用,Annexin VFITC凋亡检测试剂盒分析GPE对U2-OS细胞的凋亡诱导作用,流式细胞术分析GPE对U2-OS细胞周期的影响.结果 GPE剂量依赖性抑制U2-OS细胞的增殖,IC50为72μg·mL-1；GPE可诱导U2-OS细胞凋亡,并将U2-OS细胞阻滞在G0/G1期.结论 蛇接骨草具有抗肿瘤活性.
  • 摘要：Objective To explore the best concentration and acting time of the epidermal growth factor (ECF)on the proliferation of human retinal pigment epithelial (hRPE) cells in vitro. Methods CCK-8 assay was adopted to identify the dose-response and time-effect relationship of the EGF on hRPE cells. Results Under the same concentration of EGF, the hRPE absorbance (A value) increased with the prolongation of time. During the same time, the hRPE absorbance increased with the rise of EGF concentration. During the same time, the difference between the two groups with adjacent concentrations was statistically significant when the EGF concentration below 8 ng/mL ( P < 0.05 ) , but the difference was no statistic significance when the EGF concentration above 8 ng/mL ( P > 0.05 ). Conclusion The effect of EGF on the proliferation of hRPE cells is associated with the dose-response and time-effect relationship. The stimulation effect of EGF is gradually saturated when its concentration is above 8 ng/mL%目的 探索表皮生长因子(EGF)培养体外人视网膜色素上皮细胞(hRPE)的最佳浓度和最佳作用时间.方法 用CCK-8法观察EGF对体外hRPE增殖调控的剂量-效应关系、时间-效应关系.结果 在相同浓度EGF作用下,hRPE吸光度值(A值)随作用时间延长而增加；在相同培养时间条件下,hRPE A值随EGF浓度增加而增加,EGF质量浓度＜8 ng/mL的各相邻浓度实验组组间A值比较差异有统计学意义(P ＜0.05)；EGF质量浓度≥8 ng/mL的各相邻浓度实验组组间A值比较差异无统计学意义(P＞0.05).结论 EGF对体外培养hRPE增殖调控存在剂量-效应关系和时间-效应关系,EGF的促增殖作用在8 ng/mL以上逐渐达到饱和.
  • 摘要：CpG oligodeoxynucleotide(CpG-ODNs),which is widely used in radiotherapy,chemotherapy and the surgical treatment, can enhance treating effects, improve the patients' immunity, reduce the risk of complications and improve patients' quality of life. The basic concept,the structure,the functioning mechanism and anti - tumor effects of CpG-ODN are introduced. The application of CpG-ODN as a novel anti-tumor sensitizer in the tumor treatment with combination of radiotherapy, chemotherapy and surgical operation is specially discussed.%CpG寡脱氧核苷酸(CpG oligodeoxynucleotides,CpG-ODNs)在放、化疗及外科治疗中的应用,不仅提高了治疗效果,还能提高患者的免疫力,降低发生并发症的风险,改善患者的生活质量.本文简单介绍CpG-ODN的基本概念、结构、作用途径及其抗肿瘤免疫活性,重点介绍近几年来CpG-ODN作为一种新的抗肿瘤增敏剂,联合放、化疗及外科治疗在肿瘤治疗中的应用,并对CpG-ODN的安全性进行了分析,提出CpG-ODN作为抗肿瘤增敏剂的研究展望.
  • 摘要：传统中药罗汉果具有清热润肺、止咳、利咽、润肠通便的功效,其主要活性成分为具有葫芦烷型四环三萜结构的罗汉果皂苷.本文叙述各个罗汉果皂苷的发现,推测了罗汉果三萜皂苷的生物合成途径,分析了罗汉果皂苷与葡萄糖随生长时期的变化规律,为下一步的研究指明了方向.%Lo Han Kuo, which is also known as Siraitia grosuenoru ( Swingle) C. Jeffrey, has long been used in traditional Chinese medicine as an emollient and pulmonary demulcent for the treatment of constipation, cough and sore throat. Triterpene glycosides are the main active ingredients in Lo Han Kuo. The structures of mogrosides were elucidated. Based on the known biosynthesis of triterpene glycosides, their specific biosynthesis pathway, the whole biosynthetic process were elaborated, the various enzymes that might be involved were listed. Hie quantity of mogrosides at different growth stages of Siraitia grosuenorii (Swingle) fruits were also summarized.
  • 摘要：目的 建立超高效液相色谱(UPLC)法测定三七中人参皂苷Rg1、人参皂苷Rb1和三七皂苷R1的含量.方法 采用ACQUITY BEH C18(2.1mm×50mm,1.7μm)色谱柱；流动相为乙腈-水,梯度洗脱；流速为0.4 mL·min-1；检测波长为203 nm;柱温为35℃；进样体积为2μL.结果 三七中人参皂苷Rg1、人参皂苷Rb1和三七皂苷R1分别在0.116 8 ～1.168μg、0.123 6～1.236 μg、0.030 0～0.300 μg范围内与相应峰面积呈良好线性关系,平均加样回收率分别为100.1％、99.3％、99.9％,RSD值分别为0.4％、0.6％、1.0％(n=6).结论 较之HPLC法,UPLC法在不影响分离效果的情况下可大大提高三七中三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1的分析速度,改善分析效果；同时可减少溶剂的消耗.UPLC法可作为替代传统HPLC法的一种更为方便、快捷、可靠的方法.%Objective To establish the quantitative method of ginsenoside Rg1, ginsenoside Rb, and notoginsenoside R1 in Panax Notoginseng by ultra performance liquid chromatography ( UPLC). Methods This assay was performed on ACQUITY BEH C18(2. 1 mm × 50 mm, 1.7 μm) column with acetonitrile-water as mobile phase in gradient elution at a flow rate of 0.4 mL · min-1. And the detection wavelength was 203 nm. The column temperature was 35 ℃. The injection volume was 2 μL. Results Ginsenoside Rg1 ,Rb1 and notoginsenoside R1 in Panax Notoginseng had good linearity within the range of 0. 116 8 -1. 168,0. 123 6-1. 236,0. 030 0-0. 300 μg, respectively. And their mean recovery rations were 100. 1% ,99. 3% ,99. 9% with RSD of 0. 4% , 0. 6% , 1. 0% (n = 6). Conclusion UPLC method may greatly improve the separation efficiency and analysis speed in the case of notoginsenoside R1, ginsenoside Rg1 and Rb1 in Panax Notoginseng while reducing the solvent consumption. As an alternative of conventional HPLC,UPLC is more convenient,rapid and feasible.
  • 摘要：目的 建立测定人血浆替考拉宁的HPLC法,供血药浓度监测用,指导临床安全合理用药.方法 色谱柱为Wondasil C18柱(4.6 mm ×250 mm,5μm)；流动相为乙腈-0.05 mol/L KH2 PO4溶液(体积比28∶72,pH=3.2),检测波长为240 nm,流速为1.0 mL/min；柱温为25℃.结果 替考拉宁在4～48 μg/mL质量浓度范围内线性关系良好,检测限为2 μg/mL,方法回收率平均值为91.43％,提取回收率平均值为88.40％,日内、日间RSD值均小于6％.结论 该方法操作简单快速,准确度高,适用于替考拉宁的血药浓度测定.%Objective To establish an HPLC method for determination of teicoplanin in serum, providing the basis for monitoring clinical plasma concentrations and rational administration. Methods HPLC was performed on a C18 column (Wondasil,4. 6 mm × 250 mm,5μm) with acetonitrile-0.05 mol/L monobasic potassium phosphate 28:72 as mobile phase. The wavelength was 240 run, and the flow rate was 1. 0 mL/min, column temperature was 25℃. Results It showed good linearity in the concentration range of 4 -48 μg/mL,and the limit of quantitation was 2 μg/mL, The average method recovery was 91. 43% and the average extraction recovery was 88.40%. The inter-day RSD and intra-day RSD were both less than 6%. Conclusion The method was simple,quick and accurate,which could be used for the assay of teicoplanin in serum.
  • 摘要：目的 分析广东省2004-2010年抗精神病药物的临床应用情况,为该类药物的研究、生产、临床应用提供参考.方法 统计2004-2010年抗精神病药物在广东省的销售金额、用药频度、日用药金额并进行分析.结果 2004-2010年广东省抗精神病药物用药频度与销售金额呈逐年上升趋势,尤以非典型抗精神病药物为主,部分典型抗精神病药物仍然是用药首选.结论 2004-2010年广东省抗精神病药物的应用基本合理,不同类别药物的应用趋势对于病患合理用药、医院药品管理及生产企业决策具有参考价值.%Objective To analyze the situation of antipsychotics used in the hospitals of Guangdong Province during the period from 2004 to 2010,and to provide information for the research,production and clinical application of these drugs. Methods The antipsychotics were classified,compared and analyzed in respects of the varieties, sales (sum of money), DDDs and daily cost. Results DDDs and the sales (sum of money) of the antipsychotics had gradually risen from 2004 to 2010, especially the atypical antipsychotics. Some kinds of typical antipsychotics were still the first choice for therapy. Conclusion The application of antipsychotics from 2004 to 2010 in Guangdong Province was basically rational. It's significant for patients' rational drug-use, for the hospital drug management and for drug manufacturing enterprises to develop different kinds of antipsychotics according to the trend.

    广东药学院学报的期刊信息

    • 创刊时间：-
    • 地区：-
    • 语言：中文
    • 热门主题：-
    • 学科分类：

    广东药学院学报的获奖情况

    广东药学院学报的收录情况

    • 中国科技论文与引文数据库 (2019)
    • 中国科技论文与引文数据库 (2018)
    • 中国科技论文与引文数据库 (2017)
    • 中国科技论文与引文数据库 (2016)
    • 中国科技论文与引文数据库 (2015)
    • 中国科技论文与引文数据库 (2014)
    • 中国科技论文与引文数据库 (2013)
    • 中国科技论文与引文数据库 (2012)
    • 中国科技论文与引文数据库 (2011)
    • 中国科技论文与引文数据库 (2010)
    • 中国科技论文与引文数据库 (2009)

    广东药学院学报的投稿信息

    • 期刊官方网站：点击前往
    • 通讯地址：广州市大学城外环东路280号
    • 邮编：510006
    • 联系电话：020-39352063
    • 邮箱：gdpu2599@163.net
    • 期刊栏目：药物化学;药剂学;药物分析;中药与天然药物;药理学;临床研究与经验
    免费订阅本刊

    客服邮箱：kefu@zhangqiaokeyan.com

    京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有 出版物经营许可证

    • 客服微信

    • 服务号