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首页> 外文会议>7th China-Japan International Congress of Microbiology Shanghai Symposium-2000 4-6 August 2000 Shanghai >Typing of orientia tsutsugamushibased on 56-KDA type-specific antigen gee sequence homologies
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Typing of orientia tsutsugamushibased on 56-KDA type-specific antigen gee sequence homologies

机译：基于56-KDA型特异性抗原gee序列同源性的虫东方型的分型

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摘要

Antigenic and genetic diversity of Orientia tsutsugamushi among strains is well demonstrated by serological tests with polyclonal or monoclonal anti-bodies and by PCR-RFLP analyses of type-specific 56-kDa antigen gene. Most recent isolates from patients in Japan were classified into 4 types, JG, JP-2, Kawasaki and Kuroki, but infections of Kato and Shimokoshi types were found sometimes. Such antigenic variation depends on the diversities of an immunodominant 56-kDa antigen located on surface of the microorgansim. To further clarify the relationship between individual strains, the 56-kDa gene of each srain was amplified by PCR and sequenced in an automated nucleotide sequencer (Pharmacia), and the relationship among strains was determined by phylogenetic analysis based on the sequence homologies in the present study. Strains used in this analysis were 6 prototype strains of Gilliam, Karp, Kato, Kawasaki, Kuroki and Shimokoshi, and 50 isolates from patients, natural rodents and mites in Japan, South Korea, China, and Thailand.
机译：通过多克隆或单克隆抗体的血清学测试以及类型特异性56 kDa抗原基因的PCR-RFLP分析，已很好地证明了tsu虫东方ient菌株之间的抗原和遗传多样性。在日本，来自患者的最新分离株被分为JG，JP-2，川崎和黑木4种类型，但有时会发现加藤和Shimokoshi类型的感染。这种抗原变异取决于位于微器官表面上的免疫显性56kDa抗原的多样性。为了进一步阐明各个菌株之间的关系，通过PCR扩增每个菌株的56-kDa基因，并在自动核苷酸测序仪（Pharmacia）中进行测序，并基于本发明的序列同源性通过系统进化分析确定菌株之间的关系。研究。在此分析中使用的菌株是Gilliam，Karp，Kato，Kawasaki，Kuroki和Shimokoshi的6种原型菌株，以及来自日本，韩国，中国和泰国的50种来自患者，天然啮齿动物和螨的分离株。

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