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首页> 外文会议>The 7th international medicinal mushroom conference >Comparison of The Anti-Tumor Activity of Polysaccharides Extracted by Boiling Water and Enzyme Assistance from Ganoderma lucidum
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Comparison of The Anti-Tumor Activity of Polysaccharides Extracted by Boiling Water and Enzyme Assistance from Ganoderma lucidum

机译：灵芝的沸水和酶辅助多糖提取物的抗肿瘤活性比较

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摘要

Polysaccharides are the most important pharmacologically active constituents of Ganoderma lucidum.In this work,polysaccarides were extracted from Ganoderma lucidum with boiling water method and enzyme assistant method.The human liver hepatocellular carcinoma cell line HepG2 was used to compare the anti-tumor effect of the two kinds of extraction with MTT test.Both of these two kinds of Ganoderma lucidum polysaccarides reduced cell viability of cancer cell HepG2 in a dose-and time-dependent manner.At low concentrations,there was no significant difference in the effectiveness of L1 and L2,while at concentrations over 0.8μg/mL the difference in the effectiveness of L2 in comparison to L1 became significant.At the concentrations of 3.2μg/mL,the cancer cells were almost killed in two days.
机译：多糖是灵芝最重要的药理活性成分。本研究采用沸水法和酶辅助法从灵芝中提取多糖，并用人肝癌细胞株HepG2比较其抗肿瘤作用。这两种灵芝多糖均以剂量和时间依赖性方式降低了癌细胞HepG2的细胞活力。在低浓度下，L1和L2的有效性无明显差异。当浓度超过0.8μg/ mL时，L2与L1的有效性差异变得显着。当浓度为3.2μg/ mL时，癌细胞在两天内几乎被杀死。

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