首页> 外文会议>The 8th International Symposium on the Properties of Water, 8th, Sep 16-21, 2000, Zichron Yaakov, Israel >Metabolic Activity of Bacteria under Conditions of Low Water Activity, Luminous Bacteria as Model
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Metabolic Activity of Bacteria under Conditions of Low Water Activity, Luminous Bacteria as Model

机译:低水分活度条件下细菌的代谢活性,发光细菌为模型

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Reinhertz et al (1) were the first to correlate between bacterial viability and the in vivo luminescence of different natural and cloned luminous bacteria in low aw before and after recovery. The luminous bacteria were incubated at different concentrations of sucrose, glycerol or milk powder at different water activities. Recombinant strains of lux-carrying Salmonella showed high correlation between their ultimate level of in vivo luminescence and their viability. Using the above described approach, one can estimate the proportion of injured luminous bacteria in the population. This information can be obtained by determining the viability, as well as the level of in vivo luminescence, before and after a short recovery period in rich medium. It has been shown that "slightly injured" cells are still able to develop a considerable level of luminescence after removal of the stress causing agent by dilution, although their viability drops sharply. However, at Aw values below 0.6, both the luminescence and the viable count decrease to the same extent. This relationship was observed also with other stress conditions, such as high temperature and the presence of antibacterial agents. Another application of bacterial bioluminescence is to monitor the metabolic activity of bacteria under low temperatures. We have shown (1), that the luminescence of different luminous bacteria can be determined at temperatures as low as -12C.
机译:Reinhertz等人(1)率先将细菌生存能力与恢复之前和之后低aw的不同自然和克隆的发光细菌的体内发光之间联系起来。将发光细菌在不同水活度下以不同浓度的蔗糖,甘油或奶粉孵育。携带lux的沙门氏菌的重组菌株在其体内发光的最终水平与其生存能力之间显示出高度相关性。使用上述方法,可以估计群体中受伤的发光细菌的比例。此信息可以通过确定在富媒体中短暂恢复之前和之后的生存力以及体内发光水平来获得。已经表明,“轻度受伤”的细胞在通过稀释除去应力产生剂后仍能产生相当水平的发光,尽管它们的活力急剧下降。但是,当Aw值低于0.6时,发光率和存活数均下降到相同程度。在其他压力条件下,例如高温和存在抗菌剂,也观察到这种关系。细菌生物发光的另一种应用是监测低温下细菌的代谢活性。我们已经证明(1),可以在低至-12℃的温度下确定不同发光细菌的发光。

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