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首页> 外文会议>Academic Conference of Asian Society of Traditional Veterinary Medicine; 200610; Beijing(CN) >Immunologic Synergism with IL-2 and Effects of cCHMIs on mRNA Expression of IL-2 and IFN-γ in Chicken Peripheral T lymphocyte
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Immunologic Synergism with IL-2 and Effects of cCHMIs on mRNA Expression of IL-2 and IFN-γ in Chicken Peripheral T lymphocyte

机译：IL-2的免疫协同作用及cCHMIs对鸡外周血T淋巴细胞IL-2和IFN-γmRNA表达的影响

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摘要

Two compound Chinese herbal medicinal ingredients (cCHMIs) were prepared respectively with epimedium polysaccharide plus propolis flavone (cCHMIs 1) and astragalus polysaccharide plus ginsenoside (cCHMIs 2). In animal immune experiment, Newcastle disease vaccine was mixed respectively with two cCHMIs and IL-2 to vaccinate 15-day-old chicken in experimental groups. On days 7, 14, 21 and 28 after vaccination, the dynamic changes of serum antibody titers were tested by micro-method. In gene expression experiment, each cCHMIs, at three concentrations, was added into cultured chicken peripheral T lymphocyte. After cultivation of 7h, the expression of IL-2 mRNA in the cell, 24h, IFN-y mRNA, were determined by semi-quantitative RT-PCR assay. The results showed that two cCHMIs, whether single or cooperative injection with IL-2, could significantly raise the antibody titers, while immunologic synergisms of two cCHMIs with IL-2 were unconspicuous. cCHMIs 1 at three doses and cCHMIs 2 at high dose could remarkably promote the expression of IL-2 mRNA in chicken T lymphocyte, two cCHMIs at three doses, the expression of IFN-γ mRNA, which could explain why the immunologic synergism of cCHMIs with IL-2 were not obvious.
机译：分别用淫羊poly多糖加蜂胶黄酮（cCHMIs 1）和黄芪多糖加人参皂苷（cCHMIs 2）制备了两种复合中草药成分（cCHMIs）。在动物免疫实验中，将新城疫疫苗分别与两种cCHMI和IL-2混合，以在实验组中接种15日龄的鸡。接种后第7、14、21和28天，通过微方法测试血清抗体滴度的动态变化。在基因表达实验中，将三种浓度的每种cCHMIs加入培养的鸡外周血T淋巴细胞中。培养7小时后，通过半定量RT-PCR测定来确定细胞中IL-2 mRNA的表达24小时，IFN-γmRNA。结果显示，无论是单次注射还是协同注射IL-2，两个cCHMI均可显着提高抗体效价，而两个IL-2的cCHMI的免疫协同作用并不明显。三种剂量的cCHMIs 1和高剂量的cCHMIs 2可以显着促进鸡T淋巴细胞中IL-2 mRNA的表达，三种剂量的cCHMIs可以显着促进IFN-γmRNA的表达，这可以解释为什么cCHMIs与CCHMIs具有免疫协同作用。 IL-2不明显。

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