Expression of a Bombyx mori Tyramine Receptor in HEK-293 Cells and Action of a Formamidine Insecticide

机译：家蚕酪胺受体在HEK-293细胞中的表达及甲am杀虫剂的作用

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B96Bom was cloned from the silkworm Bombyx mori and used for stable expression in HEK-293 cells. The expressed B96Bom receptor led to an attenuation of forskolin-stimulated intracellular cAMP production in response to tyramine. The attenuation was abolished by yohimbine. Octopamine was less effective than tyramine. Tyramine inhibited [~3H]tyramine binding to the B96Bom receptor more potently than octopamine and dopamine. The acaricide/insecticide chlordimeform and its metabolite demethylchlordimeform inhibited [~3H]tyramine binding but did not show significant agonist activity. The stable expression system might prove useful for the discovery of novel ligands and bioactive compounds.

机译：从家蚕中克隆了B96Bom，并用于在HEK-293细胞中稳定表达。表达的B96Bom受体导致酪氨酸刺激酪蛋白刺激的细胞内cAMP产生减少。育亨宾消除了这种衰减。章鱼胺的效果不如酪胺。酪胺比章鱼胺和多巴胺更有效地抑制[〜3H]酪胺与B96Bom受体的结合。杀螨剂/杀虫剂杀虫二氯仿及其代谢产物去甲基氯仿二甲可抑制[〜3H]酪胺结合，但未显示出明显的激动剂活性。稳定的表达系统可能被证明对发现新型配体和生物活性化合物有用。

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《ACS Symposium Series 892; Pan-Pacific Conference on Pesticide Science; 20030601-04; Honolulu,HI(US)》|2003年|P.183-190|共8页
会议地点 HonoluluHI(US)
作者
Yoshihisa Ozoe; Hiroto Ohta; Idumi Nagai; Toshihiko Utsumi;
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作者单位

Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University, Matsue, Shimane 690-8504, Japan;

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原文格式 PDF
正文语种 eng
中图分类农业化学;
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专利

1. Amino acid residues involved in interaction with tyramine in the Bombyx mori tyramine receptor [J] . Ohta H, Utsumi T, Ozoe Y Insect Molecular Biology . 2004,第5期

机译：家蚕酪胺受体中与酪胺相互作用的氨基酸残基
2. Molecular cloning and pharmacological characterization of a Bombyx mori tyramine receptor selectively coupled to intracellular calcium mobilization [J] . Huang Jia, Ohta Hiroto, Inoue Noriko, Insect Biochemistry and Molecular Biology . 2009,第11期

机译：选择性偶联细胞内钙动员的家蚕酪胺受体的分子克隆和药理学表征
3. Insulin and 20-hydroxyecdysone action in Bombyx mori: Glycogen content and expression pattern of insulin and ecdysone receptors in fat body [J] . Keshan Bela, Thounaojam Bembem, Sanathoibi D. Kh General and comparative endocrinology . 2017,第期

机译：胰岛素和20-羟基迪克氏菌作用在Bombyx Mori中：脂肪体中胰岛素和肌肉蛋白受体的表达模式
4. Expression of a Bombyx mori Tyramine Receptor in HEK-293 Cells and Action of a Formamidine Insecticide [C] . Yoshihisa Ozoe, Hiroto Ohta, Idumi Nagai, Pan-Pacific Conference on Pesticide Science . 2005

机译：HEK-293细胞中Bombyx Mori酪胺受体的表达及甲脒杀虫剂的作用
5. Biochemical and Molecular Biological Studies on the Action of Formamidines in Synergizing Insecticidal Action of Pyrethroids and Neonicotinoids in Aedes aegypti Mosquitoes [D] . Ahmed, Mohamed Ahmed Ibrahim. 2011

机译：甲am在埃及伊蚊中拟除虫菊酯和新烟碱类杀虫剂的协同杀虫作用中的生化和分子生物学研究。
6. Bombyx mori Pupae Efficiently Produce Recombinant AAV2/HBoV1 Vectors with a Bombyx mori Nuclear Polyhedrosis Virus Expression System [O] . Qian Yu, Pengfei Chang, Xiaoxuan Liu, 2021

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7. Immunohistochemical Localization of Prothoracicotropic Hormone-Producing Neurosecretory Cells in the Brain of Bombyx mori. (prothoracicotropic hormone/Bombyx mori/monoclonal antibody/brain neurosecretory cell/neurohaemal organ) [O] . Akira Mizoguchi, Tadanori Oka, Hiroshi Kataoka, 1990

机译：Bombyx Mori大脑脑内产生孕激素激素神经细胞的免疫组化定位。（ProthoracicoTropic激素/ Bombyx Mori /单克隆抗体/脑神经疗法细胞/神经过量器官）

Expression of a Bombyx mori Tyramine Receptor in HEK-293 Cells and Action of a Formamidine Insecticide

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