• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>Advanced Biomedical and Clinical Diagnostic Systems III; Progress in Biomedical Optics and Imaging; vol.6 no.7 >High-Throughput Flow Cytometric Screening of Combinatorial Chemistry Bead Libraries for Proteomics and Drug Discovery
【24h】

High-Throughput Flow Cytometric Screening of Combinatorial Chemistry Bead Libraries for Proteomics and Drug Discovery

机译：蛋白质组学和药物发现组合化学珠库的高通量流式细胞术筛选

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

For proteomics drug discovery applications, combinatorial microbead thioaptamer libraries (one thioaptamer sequence per bead) are being created by split synthesis method, creating a "proteomics library" of protein capture beads which can be analyzed by high-throughput screening methods - in this case, flow cytometry and cell sorting. Thioaptamers, oligonucleotides with thiophosphate backbone substitutions, function like antibodies in terms of recognizing specific protein sequences but have a number of advantages over antibody libraries. These proteomics beads can then be analyzed by high-speed flow cytometry and sorted to single-bead level depending on relative fluorescence brightness of fluorescently-labeled proteins, or for a specific protein from all of the molecules of cell subpopulations being analyzed. The thioaptamer sequences on a given bead showing high affinity for that protein can then be sequenced. Alternatively, the protein-capturing beads can be analyzed by MALDI-TOF mass spectrometry for analysis of the bound proteins. The beads can be thought of as equivalent to single-element positions of a proteomics chip arrays but with the advantage of being able to much more rapidly analyze hundreds of millions of possible amino acid sequences/epitopes on the basis of thioaptamer sequence affinities to select single sequences of interest. Additionally, those beads can be manipulated and isolated at the single bead level by high-throughput flow cytometry/cell sorting for subsequent sequencing of the thioaptamer sequences.
机译：对于蛋白质组学药物发现应用，正在通过拆分合成方法创建组合的微珠硫代适体文库（每珠一个硫代适体序列），从而创建了蛋白质捕获珠的“蛋白质组学文库”，可以通过高通量筛选方法进行分析-在这种情况下，流式细胞仪和细胞分选。硫代适体，具有硫代磷酸盐骨架取代的寡核苷酸，在识别特定蛋白质序列方面的功能类似于抗体，但相对于抗体文库具有许多优势。然后可以通过高速流式细胞仪分析这些蛋白质组学珠子，并根据荧光标记蛋白的相对荧光亮度或来自所分析细胞亚群所有分子的特定蛋白，将其分类为单珠子水平。然后可以对给定珠上显示出对该蛋白高亲和力的硫代适体序列进行测序。或者，可以通过MALDI-TOF质谱分析捕获蛋白质的珠子，以分析结合的蛋白质。珠子可以被认为等同于蛋白质组学芯片阵列的单元素位置，但是具有能够基于硫代适体序列亲和力更快地分析数亿种可能的氨基酸序列/表位的优势，以选择单个珠子。感兴趣的序列。另外，可以通过高通量流式细胞术/细胞分选在单个珠子水平上操作和分离那些珠子，以用于随后的硫代适体序列测序。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号