• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>Advanced Biomedical and Clinical Diagnostic Systems III; Progress in Biomedical Optics and Imaging; vol.6 no.7 >Small and cheap: Accurate differential blood count with minimal sample volume by Laser Scanning Cytometry (LSC)
【24h】

Small and cheap: Accurate differential blood count with minimal sample volume by Laser Scanning Cytometry (LSC)

机译：体积小，价格便宜：通过激光扫描细胞仪（LSC）可以以最少的样本量实现准确的差异血细胞计数

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Aim: In patients, e.g. with congenital heart diseases, a differential blood count is needed for diagnosis. To this end by standard automatic analyzers 500 μl of blood is required from the patients. In case of newborns and infants this is a substantial volume, especially after operations associated with blood loss. Therefore, aim of this study was to develop a method to determine a differential blood picture with a substantially reduced specimen volume. Methods: To generate a differential blood picture 10 μl EDTA blood were mixed with 10 μl of a DRAQ5 solution (500 μM, Biostatus) and 10 μl of an antibody mixture (CD45-FITC, CD14-PE, diluted with PBS). 20 μl of this cell suspension was filled into a Neubauer counting chamber. Due to the defined volume of the chamber it is possible to determine the cell count per volume. The trigger for leukocyte counting was set on DRAQ5 signal in order to be able to distinguish nucleated white blood cells from erythrocytes. Different leukocyte subsets could be distinguished due to the used fluorescence labeled antibodies. For erythrocyte counting cell suspension was diluted another 150 times. 20 μl of this dilution was analyzed in a microchamber by LSC with trigger set on forward scatter signal. Results: This method allows a substantial decrease of blood sample volume for generation of a differential blood picture (10 μl instead of 500 μl). There was a high correlation between our method and the results of routine laboratory (r~2=0.96, p<0.0001; n=40). For all parameters intra-assay variance was less than 7 %. Conclusions: In patients with low blood volume such as neonates and in critically ill infants every effort has to be taken to reduce the blood volume needed for diagnostics. With this method only 2% of standard sample volume is needed to generate a differential blood picture. Costs are below that of routine laboratory. We suggest this method to be established in paediatric cardiology for routine diagnostics and for resource poor settings.
机译：目的：在患者中，例如对于先天性心脏病，需要进行不同的血球计数以进行诊断。为此，通过标准的自动分析仪，需要从患者身上取500μl血液。对于新生儿和婴儿，这是相当大的容量，尤其是在与失血相关的手术之后。因此，本研究的目的是开发一种确定样本量显着减少的血液差异图像的方法。方法：为了产生差异化的血液图像，将10μlEDTA血液与10μlDRAQ5溶液（500μM，Biostatus）和10μl抗体混合物（CD45-FITC，CD14-PE，用PBS稀释）混合。将20μl该细胞悬液填充到Neubauer计数室中。由于腔室的确定体积，可以确定每个体积的细胞数。为了能够将有核白细胞与红细胞区分开，在DRAQ5信号上设置了白细胞计数的触发器。由于使用了荧光标记的抗体，可以区分不同的白细胞亚群。对于红细胞计数，将细胞悬浮液再稀释150倍。 LSC在微腔室中分析了20μl的稀释液，并将触发设置在正向散射信号上。结果：此方法可大大减少血液样本量，以产生差异化的血液图像（10μl代替500μl）。我们的方法与常规实验室的结果之间存在高度相关性（r〜2 = 0.96，p <0.0001； n = 40）。对于所有参数，测定内差异均小于7％。结论：对于低血容量的患者（如新生儿）和重症婴儿，必须尽一切努力减少诊断所需的血容量。使用这种方法，只需要标准样品体积的2％即可生成差分血像。费用低于常规实验室的费用。我们建议在儿科心脏病学中建立这种方法，用于常规诊断和资源贫乏的环境。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号