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首页> 外文会议>Advanced Biomedical and Clinical Diagnostic Systems III; Progress in Biomedical Optics and Imaging; vol.6 no.7 >Multispectral cytometry of single bio-particles using a 32-channel detector
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Multispectral cytometry of single bio-particles using a 32-channel detector

机译：使用32通道检测器的单个生物颗粒的多光谱细胞计数

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Detecting biological particles and subsequently identifying them in a very short period of time is highly desirable, but a very difficult task. There are several pathways for developing rapid detection systems. For example, one can reduce sample size to a very small volume, and amplify cellular components by PCR technology with a view to identifying antigen-specific molecules. Alternatively, antibody-based assays allow for detection and identification of a variety of well-characterized pathogens. The system we propose utilizes flow cytometry technology to rapidly detect spectral fingerprints or organisms. However, the current limit for simultaneously detectable fluorescence signals in flow cytometry is around 12-15. Making these measurements is very complex and the necessity for advanced spectral overlap calculations creates a number of difficult problems to solve in a short period of time. Next-generation instruments can either increase the number of detectors or modify the principles of collection. If the detector system were simplified, the overall cost and complexity of single-cell analytical systems might be reduced. This requires changes in both hardware and software that allow for the analysis of 30 or more spectral signals. Further, analysis of complex data sets requires some completely new approaches, particularly in the area of multispectral analysis. This presentation describes the key components and principles involved in building a next-generation instrument which can collect simultaneously 32 bands of fluorescence from a particle in less than 5 microseconds. This would allow the analysis of several thousand bioparticles per second. The flow cytometry system based on our new detector would be designed to be portable and low cost.
机译：非常希望检测生物颗粒并随后在很短的时间内识别它们，但这是一项非常困难的任务。开发快速检测系统有几种途径。例如，为了鉴定抗原特异性分子，可以将样品尺寸减小到非常小的体积，并通过PCR技术扩增细胞成分。或者，基于抗体的测定法可以检测和鉴定多种特征明确的病原体。我们提出的系统利用流式细胞仪技术来快速检测光谱指纹或生物。但是，在流式细胞仪中可同时检测到的荧光信号的电流极限约为12-15。进行这些测量非常复杂，需要进行高级光谱重叠计算会在短时间内解决许多难题。下一代仪器可以增加检测器的数量或修改收集原理。如果简化了检测器系统，则可以降低单细胞分析系统的总体成本和复杂性。这就需要改变硬件和软件，以分析30个或更多的光谱信号。此外，复杂数据集的分析需要一些全新的方法，特别是在多光谱分析领域。本演示文稿介绍了构建下一代仪器的关键组件和原理，该仪器可以在不到5微秒的时间内从粒子同时收集32条荧光带。这将允许每秒分析数千个生物粒子。基于我们的新型检测器的流式细胞仪系统将被设计为便携式且低成本。

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