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首页> 外文会议>Advanced Biomedical and Clinical Diagnostic Systems III; Progress in Biomedical Optics and Imaging; vol.6 no.7 >Depth-resolved fluorescence of human ectocervical tissue
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Depth-resolved fluorescence of human ectocervical tissue

机译：人体宫颈组织的深度分辨荧光

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摘要

The depth-resolved autofluorescence of normal and dysplastic human ectocervical tissue within 120um depth were investigated utilizing a portable confocal fluorescence spectroscopy with the excitations at 355nm and 457nm. From the topmost keratinizing layer of all ectocervical tissue samples, strong keratin fluorescence with the spectral characteristics similar to collagen was observed, which created serious interference in seeking the correlation between tissue fluorescence and tissue pathology. While from the underlying non-keratinizing epithelial layer, the measured NADH fluorescence induced by 355nm excitation and FAD fluorescence induced by 457nm excitation were strongly correlated to the tissue pathology. The ratios between NADH over FAD fluorescence increased statistically in the CIN epithelial relative to the normal and HPV epithelia, which indicated increased metabolic activity in precancerous tissue. This study demonstrates that the depth-resolved fluorescence spectroscopy can reveal fine structural information on epithelial tissue and potentially provide more accurate diagnostic information for determining tissue pathology.
机译：利用便携式共聚焦荧光光谱技术在355nm和457nm的激发下研究了120um深度内正常和发育异常的人类宫颈组织的深度分辨自发荧光。从所有宫颈组织样本的最顶层角质化层中，观察到了具有类似于胶原蛋白光谱特征的强角蛋白荧光，这在寻求组织荧光与组织病理学之间的相关性时产生了严重干扰。而从下面的非角质化上皮层，测量的355 nm激发诱导的NADH荧光和457 nm激发诱导的FAD荧光与组织病理学密切相关。相对于正常和HPV上皮，CIN上皮中NADH与FAD荧光之间的比率在统计学上增加，这表明癌前组织中的代谢活性增加。这项研究表明，深度分辨荧光光谱可以揭示上皮组织的精细结构信息，并可能为确定组织病理学提供更准确的诊断信息。

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