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首页> 外文会议>Advanced biomedical and clinical diagnostic systems VII >Combining Optical Coherence Tomography with Fluorescence Molecular Imaging: Towards Simultaneous Morphology and Molecular Imaging
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Combining Optical Coherence Tomography with Fluorescence Molecular Imaging: Towards Simultaneous Morphology and Molecular Imaging

机译：光学相干层析成像与荧光分子成像相结合：走向同时形态学和分子成像

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摘要

Optical coherence tomography (OCT) provides high-resolution, cross-sectional imaging of tissue microstructure in situ and in real-time, while fluorescence molecular imaging (FMI) enables the visualization of basic molecular processes. There are great interests in combining these two modalities so that the tissue's structural and molecular information can be obtained simultaneously. This could greatly benefit biomedical applications such as detecting early diseases and monitoring therapeutic interventions. In this research, a new optical system that combines OCT and FMI was developed. The system demonstrated that it could co-register en face OCT and FMI images with a 2.4 × 2.4 mm field of view. The transverse resolutions of OCT and FMI of the system are both 10 μm. Capillary tubes filled with Cy 5.5 fluorescent dye in different concentrations (750nM to 24μM) under a scattering medium (1% - 2% intralipid) are used as the phantom. En face OCT images of the phantoms were obtained and successfully co-registered with FMI images that were acquired simultaneously. A linear relationship between FMI intensity and dye concentration was observed. The relationship between FMI intensity and target fluorescence tube depth measured by OCT images was also observed and compared with theoretical modeling. This relationship could help in correcting reconstructed dye concentration. Imaging of colon polyps of APC~(min) mouse model is presented as an example of biological applications of this co-registered OCT/FMI system. In conclusion, a co-registering OCT and FMI imaging system has been demonstrated. The system enables simultaneous visualization of tissue morphology and molecular information at high resolutions over a 2-3 mm field-of-view.
机译：光学相干断层扫描（OCT）提供原位和实时的组织微观结构的高分辨率横截面成像，而荧光分子成像（FMI）可以使基本分子过程可视化。结合这两种方式非常令人感兴趣，以便可以同时获取组织的结构和分子信息。这可以极大地有益于生物医学应用，例如发现早期疾病和监测治疗干预。在这项研究中，开发了一种结合了OCT和FMI的新光学系统。该系统表明，它可以以2.4×2.4 mm的视野共同配准OCT和FMI面部图像。系统的OCT和FMI的横向分辨率均为10μm。使用在散射介质（1％-2％脂质内）下充满不同浓度（750nM至24μM）的Cy 5.5荧光染料的毛细管作为体模。获得了幻像的正面OCT图像，并成功与同时获取的FMI图像进行了配准。观察到FMI强度和染料浓度之间的线性关系。还观察了通过OCT图像测量的FMI强度与目标荧光管深度之间的关系，并将其与理论模型进行了比较。这种关系可以帮助校正重建的染料浓度。提出了APC〜（min）小鼠模型结肠息肉的成像，作为该共同注册的OCT / FMI系统的生物学应用实例。总之，已经证明了OCT和FMI共同注册的成像系统。该系统可以在2-3 mm的视野内以高分辨率同时可视化组织形态和分子信息。

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  • 会议地点 San Jose CA(US)
  • 作者

    Shuai Yuan; Michael Lai; Celeste A. Roney; James Jiang; Qian Li; Hongzhou Ma; Alex Cable; Ronald M. Summers; Yu Chen;

  • 作者单位

    Fischell Department of Bioengineering, University of Maryland, College Park, MD USA 20742;

    Fischell Department of Bioengineering, University of Maryland, College Park, MD USA 20742;

    Department of Radiology and Imaging Sciences, Clinical Center,National Institutes of Health, Bethesda, MD USA 20892;

    Thorlabs Inc., Newton, NJ USA 07860;

    Fischell Department of Bioengineering, University of Maryland, College Park, MD USA 20742;

    Thorlabs Inc., Newton, NJ USA 07860;

    Thorlabs Inc., Newton, NJ USA 07860;

    Department of Radiology and Imaging Sciences, Clinical Center,National Institutes of Health, Bethesda, MD USA 20892;

    Fischell Department of Bioengineering, University of Maryland, College Park, MD USA 20742;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    OCT; fluorescence; molecular imaging; colon cancer;

    机译：华侨城;荧光分子成像结肠癌;

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