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Photoacoustic detection of induced melanoma in vitro using a mouse model

机译：使用小鼠模型在体外光声检测诱导性黑色素瘤

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摘要

Metastasis is a life threatening complex physiological phenomenon that involves the movement of cancer cells from one organ to another by means of blood and lymph. An understanding about metastasis is extremely important to device diagnostic systems to detect and monitor its spread within the body. For the first time we report rapid photoacoustic detection of the induced metastatic melanoma in mice in vitro using photoacoustic flowmetry. A new photoacoustic flow system is developed, that employs photoacoustic excitation coupled with an ultrasound transducer capable of determining the presence of individual, induced mouse melanoma cells (B16/F10) within the circulating system in vitro. Tumor was induced in mice by injecting mouse melanoma cells through tail vein into the C57BL/6 mice. A luciferase based in vivo bioluminescence imaging is performed to confirm the tumor load and multiple metastases in the tumor-induced mice, lml of blood obtained through cardiac puncture of the induced metastasized mice was treated to lyse the red blood cells (RBC) and enriched, leaving the induced melanoma in the peripheral blood mononuclear suspension (PBMC). A photoacoustic flowsystem coupled with an ultrasound transducer is used to detect the individual circulating metastatic melanoma cells from the enriched cell suspension.
机译：转移是威胁生命的复杂生理现象，它涉及癌细胞通过血液和淋巴从一个器官移动到另一个器官。对转移的了解对于设备诊断系统检测和监测其在体内的传播极为重要。首次，我们报道了使用光声流量计在体外快速光声检测小鼠诱发的转移性黑色素瘤。开发了一种新的光声流动系统，该系统采用光声激发与超声换能器相结合，能够确定体外循环系统中单个诱导的小鼠黑素瘤细胞（B16 / F10）的存在。通过将小鼠黑色素瘤细胞通过尾静脉注射到C57BL / 6小鼠中来诱导小鼠肿瘤。进行基于荧光素酶的体内生物发光成像以确认肿瘤诱导的小鼠中的肿瘤负荷和多处转移，将通过心脏穿刺诱导的转移小鼠的1ml血液处理以裂解红细胞（RBC）并富集，在外周血单核悬浮液（PBMC）中留下诱发的黑色素瘤。与超声换能器耦合的光声流动系统用于从富集的细胞悬液中检测单个循环转移性黑素瘤细胞。

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  • 来源
    《Advanced biomedical and clinical diagnostic systems X》|2012年|p.821414.1-821414.14|共14页
  • 会议地点 San Francisco CA(US)
  • 作者

    Sagar Gupta; Kiran Bhattacharya; Jessica R Newton; Thomas. P. Quinn; John. A. Viator;

  • 作者单位

    Department of Biological Engineering and Department of Dermatology, University of Missouri, Columbia, MO 65201;

    Department of Biological Engineering and Department of Dermatology, University of Missouri, Columbia, MO 65201;

    Department of Biochemistry, University of Missouri, Columbia, MO 6521;

    Department of Biochemistry, University of Missouri, Columbia, MO 6521;

    Department of Biological Engineering and Department of Dermatology, University of Missouri, Columbia, MO 65201;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 医用物理学;
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