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首页> 外文会议>Advanced microscopy techniques II >Imaging amylopectin's order in starch using 3-dimensional polarization SHG
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Imaging amylopectin's order in starch using 3-dimensional polarization SHG

机译：使用三维极化SHG成像淀粉中支链淀粉的顺序

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In minimally destructive SHG biomedical imaging (high resolution optical slicing) is greatly desirable to extract the maximum of information from the light matter interaction. Here we develop a 3-D biophysical model and a methodology, which extracts molecular information below the experimental resolution limit. Firstly, it provides the pitch angle (SHG effective orientation) of the SHG source helix of the sample. This information is used to characterize and categorize the SHG sources among them. And secondly, it provides the degree of organization of the SHG source molecules. This can be used as a quantitative imaging biomarker able to characterize the degree of organization (homeostasis) of the sample. Here we applied the model in dried and hydrated wheat starch granules. Our results show that the SHG source molecule in starch is amylopectin. We also conclude that under hydration, the amylopectin molecules are further organized but they do not change structure. This organization is reflected to the width of the pitch angles pixels' histograms' distributions. The shorter the width is, the more organized the amylopectin molecules in starch are.
机译：在破坏性最小的SHG中，非常需要生物医学成像（高分辨率光学切片）以从光物质相互作用中提取最大的信息。在这里，我们开发了一种3-D生物物理模型和一种方法，可以提取低于实验分辨率极限的分子信息。首先，它提供了样品的SHG源螺旋的俯仰角（SHG有效方向）。此信息用于表征其中的SHG源。其次，它提供了SHG源分子的组织程度。这可以用作定量成像生物标记，能够表征样品的组织度（体内稳态）。在这里，我们将模型应用于干燥和水合的小麦淀粉颗粒中。我们的结果表明，淀粉中的SHG源分子是支链淀粉。我们还得出结论，在水合作用下，支链淀粉分子被进一步组织，但它们不会改变结构。这种组织结构反映为俯仰角像素直方图分布的宽度。宽度越短，淀粉中的支链淀粉分子越有组织。

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