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Identification of Malaria Infected Red Blood Samples by Digital Holographic Quantitative Phase Microscope

机译:数字全息定量相显微镜鉴定感染了疟疾的红血球

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Development of devices for automatic identification of diseases is desired especially in developing countries. In the case of malaria, even today the gold standard is the inspection of chemically treated blood smears through a microscope. This requires a trained technician/microscopist to identify the cells in the field of view, with which the labeling chemicals gets attached. Bright field microscopes provide only low contrast 2D images of red blood cells and cell thickness distribution cannot be obtained. Quantitative phase contrast microscopes can provide both intensity and phase profiles of the cells under study. The phase information can be used to determine thickness profile of the cell. Since cell morphology is available, many parameters pertaining to the 3D shape of the cell can be computed. These parameters in turn could be used to decide about the state of health of the cell leading to disease diagnosis. Here the investigations done on digital holographic microscope, which provides quantitative phase images, for comparison of parameters obtained from the 3D shape profile of objects leading to identification of diseased samples is described.
机译:特别是在发展中国家,需要开发用于自动识别疾病的设备。就疟疾而言,即使在今天,黄金标准仍然是通过显微镜检查化学处理的血液涂片。这就需要训练有素的技术人员/显微镜专家来识别视野内的细胞,标签化学药品将附着在这些细胞上。明场显微镜仅提供低对比度的红细胞2D图像,并且无法获得细胞厚度分布。定量相衬显微镜可以提供所研究细胞的强度和相图。相位信息可用于确定电池的厚度轮廓。由于可以使用细胞形态,因此可以计算与细胞的3D形状有关的许多参数。这些参数又可以用来决定导致疾病诊断的细胞的健康状态。这里描述了在数字全息显微镜上进行的研究,该显微镜提供了定量的相位图像,用于比较从对象的3D形状轮廓获得的参数,从而识别出患病的样品。

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