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Live cell imaging with Surface Plasmon-Mediated Fluorescence Microscopy

机译：表面等离子介导的荧光显微镜对活细胞成像

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We present a new imaging technique using surface-plasmon mediated fluorescence microscopy. It uses a similar configuration as standard prismless Total Internal Reflection Fluorescence Microscopy with an additional metallic thin film. In the case of a silver thin film we show that this technique offers many advantages: distance dependence emission filter for improved signal to noise ratio and enhanced molecular detection efficiency. This technique is of particular interest in membrane and adhesion imaging. We present real time images on live cells.

机译：我们提出了一种使用表面等离子介导的荧光显微镜的新成像技术。它使用与标准无棱镜全内反射荧光显微镜类似的配置，并带有额外的金属薄膜。在银薄膜的情况下，我们表明该技术具有许多优点：距离相关发射滤波器可改善信噪比并提高分子检测效率。该技术在膜和粘附成像中特别重要。我们在活细胞上显示实时图像。

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来源
《Advanced microscopy techniques》|2009年|P.736710.1-736710.8|共8页
会议地点 Munich(DE)
作者
Karla Balaa; Viviane Devauges; Yannick Goulam; Vincent Studer; Sandrine Levgque-Fort; Emmanuel Fort;
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作者单位

Centre d'Imageries Plasmoniques Appliquees, Institut Langevin, ESPC1ParisTech, CNRS UMR 7587, Universite Paris Diderot, 10 rue Vauquelin, 75 231 Paris Cedex 05, France;

Laboratoire de PhotoPhysique Moleculaire, UPR 3361 CNRS, Universite Paris Sud, bat. 210, 91405 Orsay cedex, France and Centre de Photonique Biomedicale, Universite Paris Sud, bat. 106, 91405 Orsay cedex, France;

Centre d'Imageries Plasmoniques Appliquees, Institut Langevin, ESPC1ParisTech, CNRS UMR 7587, Universite Paris Diderot, 10 rue Vauquelin, 75 231 Paris Cedex 05, France;

Laboratoire de Neurobiologie et Diversite Cellulaire, ESPCIParisTech, UMR 7637, 10 rue Vauquelin, 75231 Paris Cedex 05, France;

Laboratoire de PhotoPhysique Moleculaire, UPR 3361 CNRS, Universite Paris Sud, bat. 210, 91405 Orsay cedex, France and Centr;

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原文格式 PDF
正文语种 eng
中图分类医用物理学;
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1. Functional Fluorescence Microscopy Imaging: Quantitative Scanning-Free Confocal Fluorescence Microscopy for the Characterization of Fast Dynamic Processes in Live Cells [J] . Krmpot Aleksandar J., Nikolic Stanko N., Oasa Sho, Analytical chemistry . 2019,第17期

机译：功能性荧光显微镜显像：无定量扫描的共聚焦荧光显微镜，用于表征活细胞中快速动态过程
2. Fluorescence Intensity Decay Shape Analysis Microscopy (FIDSAM) for Quantitative and Sensitive Live-Cell Imaging: A Novel Technique for Fluorescence Microscopy of Endogenously Expressed Fusion-Proteins [J] . Frank Schleifenbaum, Kirstin Elgass, Marcus Sackrow, Molecular Plant . 2010,第3期

机译：用于定量和敏感活细胞成像的荧光强度衰变形状分析显微镜（FIDSAM）：内源表达的融合蛋白的荧光显微镜的新技术。
3. Fluorescence Intensity Decay Shape Analysis Microscopy (FIDSAM) for Quantitative and Sensitive Live-Cell Imaging: A Noveln Technique for Fluorescence Microscopy of Endogenously Expressed Fusion-Proteins [J] . Klaus Harter Molecular Plant . 2010,第3期

机译：用于定量和敏感活细胞成像的荧光强度衰变形状分析显微镜（FIDSAM）：内源表达的融合蛋白的荧光显微镜的一种新颖技术。
4. Live cell imaging with Surface Plasmon-Mediated FluorescenceMicroscopy [C] . Karla Balaa, Viviane Devauges, Yannick Goulam, European Conference on Biomedical Optics . 2009

机译：与表面等离子体介导的荧光型谱的活细胞成像
5. Study of influenza viral infection and polymer-mediated gene delivery in live cells by fluorescence microscopy. [D] . Chen, Chen. 2009

机译：通过荧光显微镜研究活细胞中流感病毒感染和聚合物介导的基因传递。
6. Dopamine and Amphetamine Rapidly Increase Dopamine Transporter Trafficking to the Surface: Live-Cell Imaging Using Total Internal Reflection Fluorescence Microscopy [O] . Cheryse A. Furman, Rong Chen, Bipasha Guptaroy, 2009

机译：多巴胺和苯丙胺迅速增加多巴胺转运到表面的运输：使用全内反射荧光显微镜的活细胞成像。
7. Functional Fluorescence Microscopy Imaging: Quantitative Scanning-Free Confocal Fluorescence Microscopy for the Characterization of Fast Dynamic Processes in Live Cells [O] . -1

机译：功能性荧光显微镜成像：无定量扫描的共聚焦荧光显微镜，用于表征活细胞中的快速动态过程

Live cell imaging with Surface Plasmon-Mediated Fluorescence Microscopy

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